How do you do a DNase footprint assay. What did it demonstrate in regards to the UP elements binding site? 8022 -2NA 8022 ANK -31 INAP
Q: Draw the structure of 1-palmitoleyl-2-linolenyl-3-stearyl triacylglyceride
A: A triacylglyceride contains a glycerol molecule (has 3 carbon backbone) & to each carbon a fatty…
Q: Adenylylation is a post-translational modification that can regulate the Adenylyation involves the…
A: Introduction: In living organisms, hundreds of different enzyme-catalyzed reactions occur…
Q: Which of the following describe active transport or is an example of a transport of a substance…
A: Since you have posted a question with multiple questions, we will solve the first question for you.…
Q: Which of the following can be synthesized from phenylalanine? Epinephrine Melatonin y-amino butyric…
A: Phenylalanine is an essential amino acids involved in the synthesis of many important hormones and…
Q: You are investigating the effects of a new drug your lab has created. You have an intact synapse…
A: The neurotransmitter proteins are transcribed in the nucleus and translated in the cytosol following…
Q: Discuss an example of a human gene and the function of the particular protein molecule it codes for?
A: A gene is composed of sequence of DNAs, and each gene is transcribed to form an mRNA. Exons are…
Q: The intense acidification observed in the stomach lumen is energetically driven by O a parietal cell…
A: The acidic nature of gastric secretions have been observed in many animals including humans. The…
Q: Define what a hydrogen bond is and illustrate how two peptide bonds can be hydrogen-bonded to each…
A: Water molecules, DNA double-strand bindings, proteins, and other molecules all contain hydrogen…
Q: Which one of the following statements is true of enzyme catalysts? a. Their catalytic activity is…
A: Enzymes are also known by the name biological catalyst. They are so crucial that in absence of them…
Q: Our body can get pentoses from * O (A) Glycolytic pathway O (B) Uromic acid pathway O (C) TCA cycle…
A:
Q: Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that…
A: Cancer stem cells are the ones that are ones that have the ability to self renew malignant as well…
Q: What the most important difference between cellulose and other polysaccharides discussed in class…
A: Cellulose is a type of carbohydrate that is mostly present in the plants. It is generally not…
Q: A patient of 28 years old complains of pains in the spine, persistent arterial hypertension. On…
A: Hi! Since you have posted a multiple subparts questions and haven't mentioned which subparts to be…
Q: Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that…
A: Regular stem cells like hematopoietic stem cell that give birth to the entire blood cell lineages,…
Q: Question:- Based on the figure below, predict what peptide bond could be the substrate of each…
A: Proteolytic enzymes cleave the proteins at specific locations. The specificity is determined by the…
Q: Data Table 1. Compound Source Bees Annamon Coconut al Fish Garlic Lavender Lemon Mint Star Anise…
A: The International Union of Pure and Applied Chemistry (IUPAC) nomenclature of organic chemistry is a…
Q: 7. Explain the cyclin-dependent kinases, completely. 8. Describe DNA ALKYLATING AGENTS.
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: 60%-70% of bone composition
A: the inorganic composition bond means a chemical compound that does not have any C-H bond which means…
Q: . Comment on the issue's potential benefits detriments to global health using therapy.
A: Global health is a field of study, research, and application that focuses on improving health and…
Q: Neurotransmitter Glutamate GABA Acetylcholine (Ach) Dopamine General Function (a few words) Primary…
A: A neurotransmitter is a signaling molecule secreted by a neuron to affect another cell across a…
Q: Lipid anchor is myristic acid and linked to a glycine…
A: Introduction: Lipids are a heterogeneous class of compounds that are insoluble in water and…
Q: Select the correct statement about endogenous retroviruses. Take up to 8% of the human chromosomes O…
A: Retroviruses contain RNA as a part of their genome. Endogenous retroviruses in humans are an…
Q: feedback inhibition. Which step must be inhibited to regulate cholesterol synthesis?
A: The question is about the biomolecule synthesis, i.e cholesterol. What are the steps required for…
Q: A DNA molecule rich in C-G base pairs will have less hydrogen bonds between its two strands compared…
A: DNA, as well as RNA, belongs to the class of macromolecules. They are known to store genetic…
Q: You have carried out an experiment using the spectrophotometry concept. A solution containing…
A: The Biuret, Lowry and Bradford methods are used for colorimetric determination of concentration of…
Q: eptor initiate which of the following? (select all that apply) a G protein signaling mechanism…
A: G protein couple receptors are are located integrally on the cell membrane ,they generate…
Q: WASTE OF ALCOHOL DISTILLERY PLANT GIVE 2 WASTE WASTE WASTE WHEN MANUFACTURING ALCOHOL
A: Distillery plants are the ones that are widely used for the purpose of manufacturing alcohol. This…
Q: Vitamin A's two best studied forms and functions are both rhodopsin action and gene expression…
A: Vitamin A is a fat soluble vitamin. This vitamin can be reversibly converted to the aldehyde…
Q: 1.0 0 0.5 0 5 Ligand A Ligand B 10 15 20 25 Ligand Ligand D 40 45 50 55 60 30 35 [Ligand] (MM)
A: Kd is dissociation constant which is ligand concentration at half of saturation or at 0.5 theta…
Q: Question:- Use the allosteric constant (L) to explain how the Monod-Wyman- Changeux (MWC) model…
A: Monod-Wymann-Changuex (MWC) model explains protein and ligand cooperation regards to binding each…
Q: From the Lineweaver-Burke plot of an enzyme-catalyzed reaction containing 4 μM total enzyme, you…
A: Hi. Thank you for the question. As per the honor code, We'll answer the first question since the…
Q: State whether the gene is up- or down-regulated and briefly explain the reason behind. DNA lacks…
A: Genes are composed of nucleotide bases present in the genetic DNA. A genome is a double-stranded DNA…
Q: All carbohydrate metabolic pathways TCA Cycle Be sure to include all steps, intermediates, enzymes,…
A: The citric acid cycle is also kwon as "KREB'S CYCLE". The TCA takes place in the matrix of…
Q: Which of the following intermediates serve as the brain’s primary source of energy during prolonged…
A: Starvation is the stage during which there is an unavailability of food and there occurs a drastic…
Q: What is Bio Chemistry? Explain.
A: Biochemistry- Can be used for studying properties of the biological molecules.
Q: What is the bond between amino acids? lonic bond O Acidic bond O Hydrogen bond O None of them
A: Amino acids form a peptide bond to form a peptide or protein. A peptide bond is a covalent bond…
Q: НО IZ NH₂ HO
A: The given molecule is composed of carbon, hydrogen, oxygen, and nitrogen. Molecules composed of…
Q: A-DNA has a helix diameter of 25.5 Angstrom, a pitch per turn of 25.3 Angstrom, and a rise per base…
A: The A-DNA, B-DNA and Z-DNA are the commonly occurring structural conformations of DNA. The most…
Q: What type of selection does this graph show
A: Selection : is a process that causes groups of organisms to change with time in order to adapt the…
Q: Which exchange resin can be used to separate two tripeptides (MDEA and CKRF)? A. Both B and C B.…
A: chromatography technique is a biochemistry technique widely used to separate the proteins in order…
Q: Which ion concentration relationship is true when a neuron is at rest? (Select all that apply) More…
A: When a neuron is not sending a signal it is at 'rest'. During the rest state, all the voltage-gated…
Q: Elevated Na in the diet is thought to cause pathologies due Direct organ stress and elevated blood…
A: Sodium level in the body of humans should be between 135 as well as 145 milliequivalents per litre.…
Q: Which bonds are planar (cannot rotate) in a polypeptide backbone? A. Ca-C bonds B. C-N bonds C.…
A: In a peptide bond two amino acids interact to form one amide bond, by dehydration reaction.
Q: What is the mechanism for human get infected with the pathogen that causes giardiasis
A: Giardiasis is a common diarrheal disease that can be seen all over the world. The flagellate…
Q: Inhibitory postsynaptic potentials cause what type of change at the post-synaptic membrane?…
A: Introduction: Post-synaptic potentials are neurotransmitter receptors that mediate changes in…
Q: Which of the following describe active transport or is an example of transport of a substance across…
A: The basic constituent of the cell membrane is that it is a phospholipid bilayer. The different kinds…
Q: METHOD: Touch different surfaces and then wash your hands and with your bare hands place another…
A: Introduction: Hand washing is the act of cleaning our hands with soap and water in order to remove…
Q: Summarize what you have learned about different ways to change enzyme activity. complete a fishbone…
A: enzyme activity can be affected by various factors like temperature, PH, and concentration .enzymes…
Q: Which of the following statements about a plot of V0 vs. [S] for an enzyme that follows…
A: Michaelis-Menten kinetics equation Vo = Vmax[S]/(Km+[S]) Vo is reaction rate Vmax is maximum…
Q: Refer to the table below: Oil Sample Saponification Number [A] 179 [B] 260 [C] 193 [D] 185 ✓ Which…
A: Saponification number represent the number of milligrams of KOH required to neutralize the free…
Step by step
Solved in 2 steps
What did it demonstrate in regards to the UP elements binding site?
- These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and isolated Genomic DNA at 120 V for 30 minutes. Can you explain what this gel means?During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityWhat assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.
- A cloning vector map is shown below. EcoRI Bam Ban Hind P-galactosidase Amp Bam Bam EcoRI Ori C Which restriction site is best for inserting a DNA fragment for selection of chimeric plasmid containing colonies? 1) They're all equally good. 2) Hindll 3) EcoRI 4) BamHIWhich of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?Draw a gel to represent the band shift assay result for testing the following mixtures: (1) Eukaryotic DNA along(2) Eukaryotic DNA + TFs(3) Eukaryotic DNA + RNA polymerase II(4) Eukaryotic DNA + TFs + RNA polymerase II(5) Eukaryotic DNA + TFs + RNA polymerase II + nucleotidesBands for smaller molecules can be ignored
- E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into the expression plasmid pET28a (Novagen), where expression is under control of the inducible T7 promoter.Give information about the enzyme being purified, its expression in E. coli and the property being used for its purification. please provide the references for the answer.A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently linked to agarose beads to make an affinity column. The beads were incubated with a nuclear extract and the protein:beads became phosphorylated. More nuclear extract was run over the column repeatedly and then the beads were washed extensively. The bound proteins were eluted from the column and when they were separated on a SDS-PAGE gel many bands appeared. What cellular process(es) might these proteins be involved in? Options: splicing polyadenylation DNA replication a and b only a, b and cPlasmid DNA Extraction by Alkaline Lysis Why is Glacial acetic acid added to solution? What is the function of Potassium acetate in this solution?
- What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample preparation for agarose gel electrophoresis to generate a 1X buffer solution?Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells.. Hindll Sphl Sbfl Pstl BspMI Acci Hincli Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Sacl Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 oriwhat type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp DNA fragment and why?