How would a lateral flow immunoassay using breast milk to test high levels of concentrations of IgG from a maternal mother whom was vaccinated to protect her child from RSV logistically be ma
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How would a lateral flow immunoassay using breast milk to test high levels of concentrations of IgG from a maternal mother whom was vaccinated to protect her child from RSV logistically be made? Specify the importance of making this a rapid investigative device and select the test and control antibody and whyyou chose them. Present a diagram if possible.
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- Describe the principles behind direct and indirect fl uorescent antibody tests.You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamineWhy monoclonal antibodies method is better than polyclonal antibodies method in detecting specific pathogen? Explain the reasons for both selection or rejection methods..
- What is the working principle of Lateral flowimmunochromatographic assays (LFIAs)? Supported with a figure and reference plzNhich of the following enables almost all cells of the body to be recognized by an individual's immune system as "self"? O The presence of class II MHC molecules on cell surfaces The presence of ABO antigenic markers on cell surfaces O The presence of Ig D molecules on cell surfaces O The presence of class I MHC molecules on cell surfaces QUESTION 2 In a vaccine preparation, the term "attenuated" means that the agent does NOT replicate. O True O False QUESTION 3 Opsonizing protein must attached be against O protein O phagocytic O internal components if they are to effectively stimulate phagocytosis. O surfaceIn this chapter, we described co-immunoprecipitation as a method for identifying binding partners to a protein of interest. A simpler variation of this method can also be used to isolate proteins of low abundance in a complex mixture. Arrange the steps in sequential order to use this technique for this purpose. First step Last step Answer Bank The protein mixture is centrifuged, the supernatant is removed, and the pellet is washed. The antibody binds to the protein of interest. The antibody-protein complexes become insoluble. An antibody against the protein of interest is added to the mixture. Protein A beads are added to the mixture.
- 1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)Patient A, who is a recipient of USA-based poly X8650-CA vaccine for its autoimmune allergic rhinitis often visits the doctor twice a month to complain for “idiopathic colds”. Meanwhile Patient B, who is currently taking DOH pilot-used trial 1 mono E2128-PH vaccine for the same condition do not have to visit its doctor. Based on the principles of the antibody production, can you explain why patient A visits more often?
- Two newly developed vaccine candidates (A and B) are tested in mice for their ability to elicit high concentrations of anti-meningococcal antibodies that would provide mucosal as well as bloodstream protection. Also, the ideal candidate vaccine should also provide long-lasting immunity to the infection. Below is a diagram of the results from the primary immunization with both candidate vaccines. What is the predominant antibody isotype elicited by the primary immunization with these candidate vaccines? In which part of the body is that antibody primarily found?Use this information to answer the question. Human Immunodeficiency viruses, Types 1 and 2 (HIV 1,2) Antibody testing (1985) and NAT (1999) Blood donation screening for HIV-1, the causative agent of AIDS began with antibody testing in 1985. Many improvements in testing have occurred including adding the detection of a second HIV agent (HIV-2 in 1992). A chemiluminescent immunoassay (ChLIA) is used for the qualitative detection of antibodies to HIV-1 and HIV-2. A duplex nucleic acid test (NAT) was introduced for HIV-1/HCV RNA detection in 1999, and updated to include the detection of HBV DNA in 2009 (see above). The next version of NAT will include HIV-2 RNA detection (to be implemented in 2020). Donors who test antibody reactive are further evaluated by additional tests to confirm the presence of HIV antibody and to differentiate HIV-1 from HIV-2 antibodies. Tests for this purpose have included a HIV western blot, an HIV-2 enzyme-linked immunoassay and an HIV-1 and HIV-2 rapid test…A physician is typing the blood specimen belonging to an eighteen-year-old female to receive emergency blood transfusions due to an unfortunate accident. The following are the results: Anti-A Anti-B Anti-D A1 Cells B Cells 4+ - - - 4+ Due to the initial negative Anti-D result, the medical technologist decided to perform an indirect antiglobulin test. The following are the results: Anti-D Control Positive Neg Using the IAT results, should the patient be considered Rh positive or Rh negative? Justify.
