I molar extinction coefficient, &, in the Beer-Lambert Law (A =&bc) is 134,000M-1cm-1 at 630 nm for the blue dye. As a reminder, b is the path length (in cm) and c is concentration in moles/liter, M, to match the concentration units of ɛ. The path length of cells is 1.00 cm. Calculate the initial concentration of your diluted blue dye sample.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry
Section: Chapter Questions
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Q15. The molar extinction coefficient, �, in the Beer-Lambert Law Where does the absorbance come from?

I
molar extinction coefficient, &, in the Beer-Lambert Law (A =&bc) is 134,000M-1cm-1
at 630 nm for the blue dye. As a reminder, b is the path length (in cm) and c is concentration in
moles/liter, M, to match the concentration units of ɛ. The path length of cells is 1.00 cm. Calculate
the initial concentration of your diluted blue dye sample.
Transcribed Image Text:I molar extinction coefficient, &, in the Beer-Lambert Law (A =&bc) is 134,000M-1cm-1 at 630 nm for the blue dye. As a reminder, b is the path length (in cm) and c is concentration in moles/liter, M, to match the concentration units of ɛ. The path length of cells is 1.00 cm. Calculate the initial concentration of your diluted blue dye sample.
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