If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive halophile, could MSA assist in isolating one of them? If yes, why?
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If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive halophile, could MSA assist in isolating one of them? If yes, why?
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- An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated that same unknown onto the Mannitol salt agar media?The isolate you obtained appears to be very unique. Another laboratory has requested a sample of the isolate for further research. To transport the isolate, you need to transfer it from an agar plate to a transport media broth. Describe the procedure you would use.A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?
- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?What is the difference between a mixed culture and a contaminated culture if they both contain more than one type of organism ?A culture medium on which only Gram-positive organisms grow is called a(n): Question 9 options: A) acid-fast medium B) differential medium C) enrichment culture D) selective medium
- In an experiment, the bacteria were placed in dropper bottles containing glycerol as a carbonsource. Why did the culture have to be incubated for an hour in the new media?What kind of medium might you make to selectively grow a bacterium that lives in your gut?You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 9) What makes the bacteria Gram negative?10) Approximately how long would it take you to finish the entire procedure
- A pure culture was inoculated onto a Mueller-Hinton agar plate. The Kirby-Bauer procedure was performed. One of the drugs tested showed a large zone of inhibition but also had small colonies growing within this zone. Further testing showed that these colonies were not the results of contamination. Why would these colonies be present within this zone of inhibition?Suppose you observed a bacterium growing on Mannitol Salt agar (MSA) with a yellow colony appearance in the clinical microbiology lab. Name the genus and species you are most likely observing; state its most likely oxygen needs and salt requirements; and predict if this organism would grow on MacConkey agar or Colistin Nalidixic Acid agar. If you want to control the growth of this organism in a healthcare setting, predict what disinfectants would be best to use and their modes of action. Explain your reasoningYou inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and ability to breakdown lactose. What other method would you use to confirm the result of the MacConkey agar, and what result would you expect?