In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ...... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). ******* a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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In the following "gene library" cloning experiment
Digested genomic DNA
AmpR gene
TCR gene
TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI
is the unique restriction enzyme on plasmid.
A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the
genomic DNA with EcoRI restriction enzyme. After performing the cloning
experiment and obtaining colonies on a selection plate, the obtained cells will be
..... (Hint: this question is even more challenging; the PhD student was later
demoted to an MSc student).
a) resistant to ampicillin and tetracycline
b) sensitive to tetracycline and ampicillin
c) resistant to tetracycline and sensitive to ampicillin
d) resistant to ampicillin and sensitive to tetracycline
e) sensitive to ampicillin and tetracycline
BamHI
Transcribed Image Text:In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI
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