In this activity you will extract DNA from living material. Before you begin, here are some helpful suggestions. 1 Make the extraction buffer first and keep it in the refrigerator until you are ready to use it. To make the extraction buffer, add 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water to one of the plastic cups. Stir well, and refrigerate or let stand in an ice bucket. 2 Keep the alcohol ice cold until you are ready to use it. 3 Try to move through each step of the activity as efficiently as possible. In addition to DNA, smashed-up fruit also contains enzymes that chop up DNA if it sits at warm temperatures for too long. Procedure 1 Remove any of the green stems or leaves on the strawberry (if using a banana, remove the peel). 2 Place fruit into the plastic bag. Seal the bag and gently mash up the fruit with your hands for two minutes. What does this mechanical action do to the cells and the DNA?
In this activity you will extract DNA from living material. Before you begin, here are some helpful suggestions.
1 Make the extraction buffer first and keep it in the refrigerator until you are ready to use it. To make the extraction buffer, add 2 teaspoons of detergent, 1 teaspoon of salt, and ½ cup of water to one of the plastic cups. Stir well, and refrigerate or let stand in an ice bucket.
2 Keep the alcohol ice cold until you are ready to use it.
3 Try to move through each step of the activity as efficiently as possible. In addition to DNA, smashed-up fruit also contains enzymes that chop up DNA if it sits at warm temperatures for too long.
Procedure
1 Remove any of the green stems or leaves on the strawberry (if using a banana, remove the peel).
2 Place fruit into the plastic bag. Seal the bag and gently mash up the fruit with your hands for two minutes. What does this mechanical action do to the cells and the DNA?
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