Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?
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Mary Jo prepared a series of diluted samples by taking 1 mL of sample and adding it to 4 mLs of diluent. She then diluted the sample further by adding 1 mL of diluted sample to 19 mLs of diluent. What is the final dilution factor ?
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- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?
- Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample?To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial dilutions and plate 0.1 mls of each dilution. You count 40 colonies on the plate derived from the 1/10,000 dilution. What is the cfu/ml of the pondwater? Show your work and use scientific notation.Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample? Assume: 1 g = 1 mL
- A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLThe stool samples submitted by the two friends were enumerated following enrichment. The technician prepared serial dilutions. A volume of 1 mL of stool was mixed with 1 mL of diluent. From this dilution, they then took 1 mL and added it into 1 mL of diluent. The operation was repeated 5 more times. After finishing the serial dilution, 100 μ�L has been spread on different TSA plates. Following 24h incubation, the technician counted 23 colonies at the dilution 4. What is the bacterial concentration in the enriched culture? [1 mark]
- An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?You have a sample – sample 1 - that has a concentration (✗) particles. From this you take 1 mL and add it to 99 mL water to create sample 2. You then take 1 mL of sample 2 and place it in 99 mL of water to create sample 3. And lastly, you take 1 mL of sample 3 and add it to 9 mL of water to create sample 4. What is the final dilution factor in sample 4? You may use drawings if it helps you understand and explain, though not necessary.