Please help me with finding the hypotheses that are being testing in each of the three enzyme experiments(the three tables below), and predicting the results of each of the three experiments based on the hypotheses (if/then). The laboratory experiment is enzyme activity.
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Please help me with finding the hypotheses that are being testing in each of the three enzyme experiments(the three tables below), and predicting the results of each of the three experiments based on the hypotheses (if/then).
The laboratory experiment is enzyme activity.
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- The kinetics of this novel amylase were observed using lz in KI indicator to track the amount of starch at t-O min and t-1 min. They set up the following tubes and measured the absorbance after 1 minute of incubation. The reaction was stopped with HCl and 5 ml of Iz in KI indicator was added. One ml of each was used as sample in the spectrophotometer. Table 3. Calibration Curve Test tube Concentration of stock starch, mg/mL Volume of starch solution, ml Volume of distilled water, mL Volume of I, in Kl solution, mL Absorbance 1 10 0.0009 2 10 0.4 4.6 5 0.3306 3 10 0.8 4.2 5 0.5891 4 10 1.2 3.8 5 0.8409 15 10 1.6 3.4 1.0885 16 10 2.0 3.0 5 1.25720.0025 0.002 0.0015 0.001 0.0005 10 20 30 40 50 60 70 80 -0.0005 Temperature (C) 1.What trend did you observe for the rate of peroxidase compared to the temperature of the enzyme? 2.What was the optimum temperature tested for the enzyme peroxidase? 3.Did any of the temperatures tested cause the enzyme to denature? Which one? Rate (abs/sec @ 500nm)explain the results effect of temperature and pH on amylase activity Table 1 Effect of Temperature on the Hydrolysis of Starch Time (minutes) 15 °C 37 °C 65 °C 0 2 4 5 8 10 >3 >3 >3 >3 >3 >3 >3 >3 1.831 0.642 0.993 0.300 >3 >3 >3 >3 >3 >3 Table 2 Effect of Ph on the Hydrolysis of S trach Time (minutes) 3.0 7.0 11.0 0 2 4 5 8 10 >3 >3 >3 >3 >3 >3 >3 >3 >3 1.930 1.070 0.747 >3 >3 >3 >3 >3 >3
- Enzyme kinteics of amylase using l2 in Kl was used to determine starch conc at t = 0 min and t = 1 min. Tubes were set absorbance measured after 1 minute of incubation. The reaction was halted with HCl and 5 mL of I, in KI indicator was added. 1 ml of each was used as sample in the spectrophotometer. Using this table, Concentration of Volume of Volume of Volume of l2 Test tube stock starch, starch distilled in KI solution, Absorbance mg/mL solution, mL water, mL mL 1 10 0.0009 2 10 0.4 4.6 5 0.3306 3 10 0.8 4.2 0.5891 10 |1.2 3.8 0.8409 10 |1.6 3.4 1.0885 10 2.0 3.0 1.2572 What is the standard curve for the conc. of the starch?full oxidation of 2 moles of glucose produce 36ATP O 72ATP 48ATP O 106ATP OEnzyme kinteics of amylase using l2 in KI was used to determine starch conc at t = 0 min and t = 1 min. Tubes were set absorbance measured after 1 minute of incubation. The reaction was halted with HCl and 5 mL of I, in KI indicator was added. 1 ml of each was used as sample in the spectrophotometer. Using this table, Concentration of Volume of Volume of Volume of l2 Test tube stock starch, in KI solution, Absorbance starch solution, mL water, mL distilled mg/mL mL 1 10 5 0.0009 2 10 0.4 4.6 0.3306 3 10 0.8 4.2 0.5891 4 10 |1.2 3.8 0.8409 5 10 1.6 3.4 1.0885 6. 10 2.0 3.0 1.2572 What is the standard curve for the conc. of the starch?
- I I 1 1 Testa tabes dm 3 4 5 6 distilled Water 15 10 5 O 6 amylase enzyme absorbance 0.00 0.031 0.043 0:094 0151 0.147 5 5 10 5 O O 5 5 5 3. In this experiment, what does reaction rate represent (what is it a proxy for)? What does a high absorbance value indicate? What does a low absorbance value indicate? What does an ? absorbance value of zero indicate I 1 1 1 I I 1 I I I 1 I Iexplain the results of this experiment effect of temperature and pH on amylase activity Table 1 Effect of Temperature on the Hydrolysis of Starch Time (minutes) 15 °C 37 °C 65 °C 0 2 4 5 8 10 >3 >3 >3 >3 >3 >3 >3 >3 1.831 0.642 0.993 0.300 >3 >3 >3 >3 >3 >3 Table 2 Effect of Ph on the Hydrolysis of S trach Time (minutes) 3.0 7.0 11.0 0 2 4 5 8 10 >3 >3 >3 >3 >3 >3 >3 >3 >3 1.930 1.070 0.747 >3 >3 >3 >3 >3 >3Handwritten Identify the molecule names, enzyme name, enzyme classification and change in reaction(for glycolysis pathway)
- Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organismsA solution containing 0.00001 M ATP has transmission 70.2\% at 260 nm in 1 cm cuvette calculate the transmission of solution in a 3 cm cuvette33333333333333333333333 Using the table below, differentiate the effect of two varying pH levels (as indicated by by the color) to the amylase enzyme. How does pH level affects the enzymatic reaction (enzyme-substrate complex)? Tube 1 Tube 2 Tube 3 Tube 4 Ingredients StarchAmylaseBuffer pH 7 StarchAmylaseBuffer pH 2 MaltoseWaterBuffer pH 7 StarchWaterBuffer pH 7 Color (1) (2) Orange Blue