Question 1 Listen Tina Is the mother of Violet, Jose, Kelly and Paul. Ramone is a potential father. You are trying to determine which if any children could be the offspring of Ramone Tina Ramone Violet Jose Kelly Paul == =- Gene 1 Gene 2 You analyze 2 genes Gene 1 and Gene 2 known to have variable numbers of repeats. After PCR and gel electrophoresis, you get the results shown above. Based on these results you conclude that Violet a) Must be the offspring of Ramone and and Tina b) Can not be the offspring of Ramone and Tina c) Could be the offspring of Ramone and Tina
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- Question 7 Review DNA sequencing and cloning tools. The direct manipulation of genes is called genetic engineering DNA sequencing nucleic acid hybridization gene cloning O recombination DNANikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a PCR technique illustrated below (Figure 2). Deduce FOUR (4) possible reasons why this method is utilized rather than the conventional PCR technique. +1 A 422 318 275 232 181 109 B * Marker PCR with all four primer pairs in a single tube 371 275 202 141 Figure 2 ᎠNikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a PCR technique illustrated below (Figure 2). Deduce FOUR (4) possible reasons why this method is utilized rather than the conventional PCR technique. 422 318 275 232 181 109 Marker 01 PCR with all four primer pairs in a single tube 371 275 202 141 Figure 2
- | Choose J [Choose] repetitive DNA transposable elements transposon retrotransposon pseudogenesJoe is heterozygous for a dominant genetic disease. His wife, Jenny does not have the disease. They have 5 children of which 2 (Jim and Jan) have the disease and 3 (Jose, Jerry and Julie) do not. DNA from each individual is isolated and analyzed by PCR an gel electrophoresis. 3 variable markers are studied. The results for each marker are shown here. The numbers to the left of each gel represent size in hundreds of bases Jim Jose Marker 1 Marker 2 Marker 1 Jenny Joe Marker 3 Jan Jerry Julie Jim Jose Marker 2 Jenny Joe Jan Jerry Julie Jim Jose Marker 3 Jenny Joe Based on these results, which marker is most likely associated with the condition? Jan Jerry JulieUsing the designed forward and reverse primer from Question 23-30, you performed PCR to amplify the CO1 gene of S. tawilis. Then, you verified the PCR product using the Agarose Gel Electrophoresis. Here is the result of the AGE. M-Marker L1-PCR Product ML1 You noticed that there are many bands in your PCR product. What does this indicate? Choose the best answer. The primers were not specific to CO1 gene. The annealing temperature is too high. The templated DNA is not enough. There is a formation of primer dimers. The primer length is too short. The melting temperature is too high.
- Question 2. You have a wild-type strain of E. coli with the genotype A B C D EF You introduce an F+ plasmid into your wild-type strain and isolated a few Hfr derivative strains that you call Hfr1, Hfr2, and Hfr3. You are studying several new genes in E. coli with interesting phenotypes. You obtain a multiply mutant strain with chromosomal genotype: a b c d e f a) You mate each Hfr strain to your multiply-mutant strain in a separate experiment. At various times you interrupt the matings and plate the bacteria under conditions in which only the recipient strain can grow. You obtain the following earliest-time-of-entry data, in minutes: Gene Hfr1 Hfr2 Hfr3 A B C D E F 11 13 7 I 26 16 11 9 5 31 15 27 31 11 Draw a map of these genes that is consistent with the data, including all the genes and Hfr origins, the distances between them (in minutes), and the direction of transfer of each Hfr.b) Among the progeny from the Hfr3 mating above, you find one that has the genotype: Abc de F Draw out the gene transfer and crossover(s) that produced this outcome: c) Among the progeny from the Hfr3 mating above, you find one that has the genotype: A B c d e f Draw out the gene transfer and crossover(s) that produced this outcome:Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…
- QUESTION 49 You have discovered a very small amount of DNA from an ancient organism that you want to save and study. What is the very first thing you should do to allow you to study this DNA in the lab? O a. Insert the DNA into a vector Ob.RT-PCR Oc. Gel electrophoresis. O d.PCR Click Save and Submit to save and submit. Click Save All Answers to save all answers. Save All Answers Save and Submit ScieCopy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?QUESTION 7 Primers are needed to start a PCR reaction True O False QUESTION 8 Restriction enzymes specifically recognize and cut short sequences of DNA called introns. O exons. O sticky ends. restriction sites. QUESTION 9 The DNA profiles used as evidence in a murder trial look something like supermarket bar codes. The pattern of bars in a DNA profile shows O the order of genes along particular chromosomes O the order of bases in a particular gene O the presence of differently-sized fragments of DNA O the presence of dominant or recessive alleles for particular traits O the number of chromosomes and whether any are damaged