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- Question 7 A unique 5'CCA terminal sequence is found in all RNAS to be able to carry the correct amino acid to the ribosome. A True B) FalseQuestion 23 The MRNA produced when the following sense strand DNA sequence is transcribed is: 5'- ATGGATGATTGGGAAATT-3' O 5- UUAAAGGGUUAGUAGGUA -3' O 5- AUGGAUGAUUGGGAAAUU -3' O 5- UACCUACUAACCCUUUAA -3' O 5- TACCTACTAACCCTTTAA -3' O 5- AATTTCCCAATCATCCAT -3'Question 33 RNA polymerase IlI: A is located in the nucleoplasm and transcribes the protein-encoding genes through mRNAs. B transcribes tRNA genes and protein transport genes. c) transcribes the 55 RNA genes. D) transcribes RNA genes associated with TRNA processing.
- QUESTION 16 CRISPR in nature evolved as a "bacterial immune system" or "genetic vaccine" to target infecting viruses. What aspect of the system can we now manipulate so that you can promote *more* transcription of a desired gene? O A nuclease deficient cas9 a single amino acid mutation in Cas9 o edit the PAM sequence supply a DNA template with the cas9/guide RNA use a special guide RNAQuestion 47 (a) Use the following figure to determine the changes to the amino acids that correspond to the normal and mutated DNA sequences. Normal DNA sequence: 3 CAT TCA AAC ATT 5 Mutated DNA sequence: 3 CAT AGT GAG GTC 5 (Hint: Write the mRNA first, then identify the amino acids.) (b) What type of mutation is shown? First base of codon U C A G U UUU UUC UUA UUG CUU CUC CUA CUG AUU AUC AUA AUG GUU GUC GUA GUG UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA Met ACG GCU GCC GCA GCG Phe -Leu Leu lle Second base of codon A C Val -Ser Pro Thr Ala UAUTYT туг UAC UAA UAG CAU CAC CAA CAG. His Gin AAU AAC Asn AAA GAUT GAC GAA GAG Asp G AAGLYS AGG. GGU GGC GGA GGG Glu UGU UGC UGA UGG CGU CGC CGA CGG Bys A Trp G U C -Arg AGU AGC AGA аса за Ser Arg U C Gly AG A U C A G U C A G Third base of codonQUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.
- Question 26 The initial event in the conversion of an hnRNA to the mature RNA which leaves the nucleus is: A transesterification reactions in lariat formation. B splicing together of the exons. © addition of a poly(A) tail to the 3'-end of the transcript by poly(A) polymerase. D) capping the 5'-end of the transcript with a guanylyl groupQuestion 45 Once the RNA primers are replaced the fragments in the lagging strands are sealed by DNA pol I. A True B) FalseQUESTION 22 If polynucleotide phosphorylase (PNP) synthesizes random RNA when given a 50-50 mixture of G and A substrates, and this RNA is used to program an in vitro (cell free) translation system, what amino acids would be incorporated? Second letter G UUU UCU) UCC UCA UCG UAU UAC Tyr UGU UGC. Phe UUC U UUA Ser UAA Stop UGA Stop A UAG Stop UGG Trp G UUGLEU CAU His CUU CUC CUA CUG CCU CC CCA CCG CGU CGC Arg U CAC CAA Leu Pro CGA CAGJGin CGG AUU ACU ACC ACA AAC FAsn AAA AAU Asn AGU AUC le A AUA AGC Ser Thr AAG Lys AGA GAUASP AUG Met ACG JArg AGG G GUU GCU) GACA GAA Glu GAGJ GGU] GGC GGA GGG GUC G GUA GCC Ala GCA Val Gly GUG GCG First letter DUAG Third letter
- Question 21 There are no aminoacyl-tRNAs that will go to the A site of the ribosome when UGA is the codon. A) True B) FalseQuestion 4 "In this mutation, the AGC is transcribed into UAG. " O missense mutation O nonsense mutation O silent mutation O frameshift mutationQUESTION 7 After you graduate, you are working at a laboratory that identifies a novel protein called Leprechaun. To determine in which organelles Leprechaun is found, you decide to express the Leprechaun gene in HeLa cells using a plasmid. Since you have generated an antibody that recognizes an epitope within the Leprechaun protein, which technique will you use to identify its localization within the HeLa cells? OA. SDS-PAGE O B. Immunocytochemistry O C. Western blot OD. Transmission electron microscopy (TEM) OE. Immunohistochemistry QUESTION 8 Which of the following statements regarding electron microscopy is TRUE? OA. White light is utilized to illuminate the sample for imaging in all types of electron microscopy. OB. Electrons pass through the object being examined in transmission electron microscopy (TEM). OC. In scanning electron microscopy (SEM) electrons, electrons bounce off the surface of the sample. OD. Electron microscopy can be used to view structures as small as 2 nm in…