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Genetics Question 4
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- QUESTION NO. 1 A transition mutation A. occurs when a purine is substituted for a pyrimidine or vice versa. B. results from the insertion of one or two bases into the DNA chain. C. is most frequently caused by chemicals (like acridine) that intercalate into DNA. D. results from substitution of one purine for another or of one pyrimidine for another. E. always is a missense mutation QUESTION NO. 2 Degeneracy of the generic code denotes the existence of A. multiple codons for a single amino acid. B. codons consisting of only two bases. C. base triplets that do not code for any amino acid. D. different systems in which a given trip let codes for different amino acids. E. codons that include one or more of the unusual bases. QUESTION NO. 3 Replication A. requires that a phosphodiester bond of the incoming dNTP be hydrolyzed in order to be added to the growing chain. B. uses 5' to 3' polymerase activity to synthesize one…QUESTION 22 The DNA sequences that are most conserved between human and mouse would most likely be located in: A Highly-repeated sequences, such as microsatellite regions B Highly repeated sequences, such as Alu sequences C Moderately-repeated non-coding sequences D Coding regions of single-copy genesQuestion 1. The nitrogenous base content of a sample of DNA was found to be 34% adenine. Determine the amounts of the other three bases in this sample.
- QUESTION NO. 1 Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. The study of such patients has enhanced our knowledge of DNA repair because XP is caused by defective DNA repair nucleotide excision repair. (A variant, XP-V, is deficient in postreplication repair.) In nucleotide excision repair A. removal of the damaged bases occurs on only one strand of the DNA. B. only thymine dimers generated by UV light can be removed . C. the excision nuclease is an exonuclease. D. a single multifunctional enzyme carries out the repair process. E. only the damaged nucleotides are removed. QUESTION NO.2 Homologous recombination: A. occurs only between two segments from the same DNA molecule. B. requires that a specific DNA sequence be present. C. requires one of the duplexes undergoing recombination be nicked in both strands. D. involves a…QUESTION 24 During lagging strand synthesis of DNA, Okazaki fragments are linked together by ___________. DNA polymerase I Primase Beta clamps DNA LigaseQuestion 1: tRNA and amino acyl tRNA synthetases Part a: How many codons encode the amino acid Isoleucine (Ile, I)? Part b: How many codons encode the amino acid Valine (Val, V)? Part c: How many codons encode the amino acid Methionine (Met, M)? Part d: How many tRNAs decode the Ile codons? Part e: How many tRNAs decode the Val codons? Part f: How many tRNAs decode the Met codon?
- QUESTION 23 What is the most stable nitrogenous base pairing? A. A-T B. G-C C. G-A D. C-T E. A-UQUESTION 25 During lagging strand synthesis of DNA, the RNA primers are replaced with DNA by __________. DNA Polymerase I DNA Polymerase II DNA Polymerase III PrimaseQUESTION 25 What is the most common type of DNA sequence present in eukaryotic genomes? A. Repetitive DNA sequences B. Minisatellites C. Exons of genes encoding proteins D. Introns of genes encoding proteins
- Question Number 3: Write the sequence of the mRNA molecule synthesized from a DNA template coding strand having the sequence 5’ – ATCGTACCGTTA – 3Question 3 (1 pt): Which positions in adenine and guanine have the potential to form hydrogen bondsbut are not involved in Watson-Crick base pairing? What might this be used for?1 of 2Question #2 To isolate DNA from cells, you must disrupt the cell Therefore, you treated your cells with a lysis buffer that contains a detergent. You also heated the cells and vortexed them during this time. What chemical property does the detergent in the lysis buffer have that helps disrupt a cell membrane? Briefly explain your answer. How does vortexing and heating your samples aid in disrupting the membrane?