Q: Why are some of the detectors listed unsuitable for HPLC?
A: HPLC is applicable for the use with solvent that has UV absorbance. A UV detector cannot be used…
Q: Calculate the Re value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot…
A: Given Distance moved by solvent = 14.5 cm Spot of Compound moved from the origin = 6.1 cm Rf =…
Q: Which of the following results from an in-line HPLC mobile phase filter becoming plugged with…
A: Correct option is B.
Q: kindly prepare an assignment on Application of HPLC in Drug Analysis: one page show name of the…
A: In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form,…
Q: What is the type of column used in chromatography (HPLC) and it's applications and mechanism
A: It is as follows
Q: How can you vary the retention factor and selectivity factor?
A: Retention factor is a variable which indicates the time duration a component spends in the…
Q: a. What does ECD mean. b. Discuss how column temperature influences separation in a chromatography…
A: “Since you have posted a question with multiple sub-parts, we will solve the first three subparts…
Q: On a 125cm retention times Long column. operated at look these. are obtained. octane 1.43 Air peak…
A: Since you have asked multiple questions we will answer the first one for you. To get the remaining…
Q: Which of the following is/ are adsorption chromatography: Select one: a. TLC O b. Column…
A: Adsorption chromatography Adsorption Chromatography involves the analytical separation of a…
Q: order to execute paper chromatography, which of the following stages is required? I. Load the…
A:
Q: Before developing a TLC plate you should _______. a. Make sure to mark the origin with a pen b.…
A: While developing a TLC plate, we should always use a pencil (not pen) because: Pencils are cheap and…
Q: How many theoretical plates produce a chromatography peak eluting at 12.83 min with a width at…
A: The no. of theoretical plates can be calculated as: Where N is the no. of theoretical plates, tr is…
Q: A Student attempled to Rerin a product mxture by calumo Chromatography with a silica gel Columo and…
A: The separation of mixture is generally carried by chromatography technique. There are two phases…
Q: (5). Write short notes on molecular exclusion chromatography with representative picture.
A: Molecular exclusion chromatography, otherwise called Size-exclusion chromatography (SEC), is a…
Q: 2. A TIc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography…
A:
Q: spot of black ink was placed on a strip of filter paper. The chromatogram was developed using an…
A:
Q: Why is splitless injection used with purge and trap sample preparation?
A: The collection of all of the analyte from the unknown and to inject whole of the analyte into the…
Q: Which of the following is the correct flow of the mobile phase in HPLC? Solvent degasser>Guard…
A: The mobile phase in HPLC is used to transport the sample via seperation column and there by detector…
Q: When the use of 2D Thin layer Chromatography is better than normal TLC? Please shortly answer at…
A:
Q: What are the mandatory components required for column Chromatography? Please shortly answer at your…
A: Columns chromatography is used for separation of mixtures By using stationary and mobile Phase.
Q: (a) Total Hardness Volume of EDTA used (V2) X 1000 ppm Volume of Sample (V1) 9 ×1000 3 180 ррт 50…
A: The water containing dissolved salts of calcium and magnesium is called hard water. Hard water is…
Q: if air can degrade a column and cause TC detector filaments to burn out, why do we use an air peak?
A: Interpretation - If air can degrade a column and cause TC detector filaments to burn out, why do we…
Q: The mode in liquid-liquid chromatography is all of the above a O reversed phase b O size exclusion…
A: All the Above.
Q: conclusion about chromotography of hexane
A: Chromatographic techniques were used for the separation of substances based on their…
Q: (10) In calculating r, values during tlc analysis the distance run by the sample and by the solvent…
A: Various techniques are used to analyze the compounds present in a mixture. Chromatography is one of…
Q: Vhat criteria should you use to determine the solvent for dissolving a sample for TLC analysis? Vhat…
A: the solvent should be 2-3 mm deep so that the spotted sample and the line are not submerged this…
Q: High performance liquid chromatography can separate eluates even if they are not present in the UV…
A: The actual separation of each component in a sample is always carried inside a column and to be able…
Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…
A: Thin layer chromatography.
Q: Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move…
A:
Q: SFC combines important advantages of gas and liquid chromatography. It is especially useful for…
A: The chromatography is the separation technique used for identification and separation of compounds…
Q: A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of…
A: Chromatography is an analytical and separation technique which is used to separate the components…
Q: what ccan be a cause of not getting a baseline separation when it comes to chromatography? if it is…
A: chromatography It is the separation technique that involves the principle of affinity of the…
Q: (i) Pure RSOP86 powder has a melting point of 130 °C and a degradation temperature of 340 °C. With…
A: TGA thermograms is an analytical technique which is used to measure the weight loss of substances…
Q: Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table…
A: The function of detectors used in high-performance liquid chromatography is to monitor the mobile…
Q: The total retention time of dodecane on a 15 m x 0.25 mm i.d. DB-5 GC column was 8.0 min. The column…
A: Given: Number of theoretical plates=N=25600Retention time=tR=8.0 min
Q: The HPLC chromatogram below was collected using a reverse-phase column with a length of 10 cm.…
A: Resolution is an important HPLC performance indicator usually assessed by how quickly and how…
Q: What will be the appearance of a TLC plate if a solvent of too low polarity is used for the…
A: In TLC, stationery phase is used is polar in nature. components or analyte that has to seperate, has…
Q: In supercritical-liquid chromatography, methanol is usually added as a modifier .a O all of the…
A: as per my opinion it is option- D
Q: I) Choose the Correct Answer from the Multiple-Choice List (10) 1. Which of the following is not a…
A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…
Q: Compare gradient and isocratic mode in HPLC.
A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.
Q: select
A: We know that A detector is a device that converts an amount of radiation into some other measurable…
Q: Use the data presented in Figure 2 as basis for your answer. The TLC plate used is 5 cm by 10 cm.…
A: According to the given TLC plate, Commercial analgesic dot A, show two signals at 1.5 cm and 3.9…
Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.5 cm and the spot…
A:
Q: Two components in an HPLC separation have retention times that differ by 15 s. The first peak elutes…
A: The base number of hypothetical plates under given conditions ought to be resolved to utilize the…
Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system
A: Basically there are following two reasons for using filtration during HPLC sample preparation are…
Q: (7) Once you put the spotted TLC plate into the solvent, you will see the solvent begin to move up…
A: Since you have asked multiple questions, we will answer first one for you. For remaining questions,…
Q: Should you increase the diameter or length of the chromatography colum for increasing throughput?
A:
Q: Sort the following molecules in order of increasing Rf value assuming a normal-phase silica TLC…
A: In thin layer chromatography is a technique to separate different components in a mixture based on…
Q: How do you weigh hydgroscopic samples?
A: Hydgroscopic samples contain moisture. Therefore it is measured in digital analytical balance.
Q: According to given TLC plate below which of the following statement(s) is/ are false ? |- Rf value…
A: It is given a TLC plate in which the solvent travels a distance of 0.75 (approximately), and…
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- Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC system to a mass spectrometer is a much more difficult task. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC with mass spectrometry.How can the selectivity factor be manipulated in (a) GC and (b) LC?1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
- Different from GCMS, why is an interface needed when coupling HPLC with MS?Why is it more difficult to optimize k' and α in HPLC than in GC hand written please.6. This is the TLC plate for the solvent: Ethanol 50% / ethyl acetate 2. 50% V is crystal violet, G is bromocresol green and R is methyl Red. Determine the Rf for each one (Report to 2 # after the decimal) HTML Editor B IUA A 工E三ョ x x 三三 ▼ 目 回 深 T 1 12pt Paragraph 7. 3. 4 5 1. #4
- 100 80 60 20 % of base peak 30 40 80 V (cm¹) M**#103 120 160 m/e 200 Mass Spectrum C4H9NO₂ 240 280 21 No significa absorption abHPLC VFA analysis - why do I get so different peaks for very similar samples?The Rf calculated for the TLC separation of a component of two-component mixture is determined to be equal to 2.3. Does this represent good separation or not? Explain.
- EXP 3 + X M+ 12/14 CHM-202 Lab 3 Chromatographic Separation Rev2 G6-2022 3. How would the Rf values change if you used pure dichloromethane (no methanol) as the mobile phase for the TLC separation of curcumin? What if you used pure methanol (no dichloromethane)?100 Transmittance 16T Relative Intensity 1. An oily liquid having a boiling point of 191°C and a melting point of -13°C. What is the structure of this compound? Major lons in the MS 50 (small) Major IR Absorptions 3066 cm-1 2230 (str) 1599 76 103 (base peak and molecular ion) 104 (=8% of base) 1492 1448 758 688 mmm T 1599 1492 1500 liquid film sample 0+ 4000 100 80 60 40 20 0 10 1 20 3066 3000 30 40 pump 2230 2000 50 50 vavenumber cm 60 m/z 70 1448 76 80 90 1000 758 680 103 100 110 50016:24 5 ê ☺ I o The glucose concentrations in a patient with mildly elovated glucose lovels were determined by a spectrophotometric analytical method and wore recorded in mot, as tolows 700 775 7eg 774 T77 T80 and 758 Caculato the standard devialion OA 114 OR 135 OC124 O. 129 OL 222 Add a caption... > Status (Custom) +