РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3' Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete the following table for PO and P3Mod to verify that these two primers adhere to the general criteria required for primer design: CHARACTERISTIC CRITERIA FOR AN EFFECTIVE PRIMER 1: PO PRIMER 2: P3MOD PRIMER Length of primer Minimum length 17 bases; between 17 - 30 bp 19 nt 20 nt Base composition G+C content 40-60% Tm of primers Tm 60-75°C The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C); where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and (G + C) is the sum of the G and C residues in the oligonucleotide.
Fundamentals of Sectional Anatomy: An Imaging Approach
2nd Edition
ISBN:9781133960867
Author:Denise L. Lazo
Publisher:Denise L. Lazo
Chapter1: Introduction
Section: Chapter Questions
Problem 14RQ
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Transcribed Image Text:РО
P3mod
5' GAGTTTGATCMTGGCTCAG 3'
.5' ATTAGATACCCTDGTAGTCC 3'
Transcribed Image Text:Considering only the PO and the P3Mod primers from Table 1 in Wilson et al. (1990), complete
the following table for PO and P3Mod to verify that these two primers adhere to the general
criteria required for primer design:
CHARACTERISTIC
CRITERIA FOR AN EFFECTIVE
PRIMER 1: PO
PRIMER 2: P3MOD
PRIMER
Length of
primer
Minimum length 17 bases;
between 17 - 30 bp
19 nt
20 nt
Base
composition
G+C content 40-60%
Tm of primers
Tm 60-75°C
The "Wallace rule" for calculating melting temperature TM: Tm = 2(A + T) + 4(G + C);
where Tm is in °C and (A + T) is the sum of the A and T residues in the oligonucleotide and
(G + C) is the sum of the G and C residues in the oligonucleotide.
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