Scatchard analysis rearranges the basic equation for protein-ligand binding to enable a linear fit of experimental data for bound to free ligand concentration ([L]bound/[L]free) as a function of bound ligand concentration ([L]bound). This enables extraction of the dissociation constant KD and the total protein concentration, [P]total, from the plot. Given the standard equation for a line, y = mx + b, where x is the abscissa and y is the ordinate, express KD in terms of the fitting parameters m and b.

Scatchard analysis rearranges the basic equation for protein-ligand binding to enable a linear fit of experimental data for bound to free ligand concentration ([L]bound/[L]free) as a function of bound ligand concentration ([L]bound). This enables extraction of the dissociation constant KD and the total protein concentration, [P]total, from the plot. Given the standard equation for a line, y = mx + b, where x is the abscissa and y is the ordinate, express KD in terms of the fitting parameters m and b.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter4: Calculations Used In Analytical Chemistry

Section: Chapter Questions

Problem 4.2QAP

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