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- Discuss how sample concentration is determined using the standard addition method. What limitation does it overcome?A plot of absorbance against concentration is called the calibration curve. True or FalseTrue or False. 1. The absorbance of a sample is equal to the negative logarithm of the transmittance. 2. Winkler method of dissolve oxygen determination is an example of iodometry. 3. A diazo compound is produced when nitrite is reacted with sulfanilamide in acidic solution. 4. Atomic Absorption Spectroscopy can be used to analyze the functional groups of organic substances. 5. A diode array spectrometer use an absorption or interference filters for wavelength selection and a photoelectric device for measuring radiant power. 6. The oxidation state for the manganese in the brown solid which precipitate in Winkler method is +3 7. In Winkler method, the iodine produced is oxidized during sodium thiosulfate titration to iodide ion. 8. When the pH of the solution is adjusted to pH greater than 12, the EDTA reacts with magnesium ions only, because calcium precipitates as calcium hydroxide.
- It is never necessary to take more than one composite sample per field. True Or FalseWhy is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)? What would happen if you put too much of a compound in the cuvette during the spectral measurement? Why is it important to avoid any scratch on the cuvette? Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not?A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
- A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. What is the concentration of KMNO4 if its absorbance is 0.678? Table 1. Data for the Standard Calibration Curve KMNO4 Standard Solution (mol/L) Absorbance at 525 nm 0.000400 0.112 0.000800 0.295 0.000120 l0.443 0.000160 0.701 0.00200 0.932 O 0.00173 M 0.00194 M O 0,00189 O 0.00155 MA standard addition calibration curve was generated by plotting measured absorbances at 280nm versus the concentration of standard added. Select the equation that should be used to calculate the standard error in the concentration of an unknown determined using this calibration curve.A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
- 1. at what wavelength was Pb determined? 2. What is the slope of the calibration curve? 3. What is the y-intercept of the calibration curve? 4. The researcher determined the Pb content of water sample from a river suspected to be contaminated with Pb from a nearby factory by complexing with cyanidine and the absorbance measured was 0.405. What is the lead content of the water sample?Make a standard calibration curve wherein the x-axis is the total concentration of standard 1 to 6 and the y axis is their corresponding corrected absorbance ..(Note never include the absorbance reading of the unknown sample when making a standard curve) Absorbance Reading Test Tube / Standard Glucose Concentration (mg/mL) Initial Absorbance Corrected Absorbance Blank 0.00 0.045 0.000 Standard 1 1.00 0.102 0.057 Standard 2 2.00 0.236 0.191 Standard 3 4.00 0.697 0.652 Standard 4 6.00 1.104 1.059 Standard 5 8.00 1.385 1.340 Standard 6 10.0 1.915 1.870 Unknown 2.42 0.361 0.316 Data Set for Quantitative Analysis Tube No. ABSORBANCE TRIAL 1 TRIAL 2 TRIAL 3 TRIAL 4 TRIAL 5 TRIAL 6 1 0.041 0.044 0.046 0.046 0.046 0.046 2 0.102 0.103 0.103 0.102 0.102 0.102 3 0.236 0.235 0.240 0.235 0.237 0.235 4 0.703 0.698 0.696…Calculate the concentration of the unknown MnO4 sample, given the following standard addition experiment. Flask 1: A 2.00 mL aliquot of an unknown MnO4 sample was diluted to 100.0 mL and the absorbance measured to be 0.225. Flask 2: A 2.00 mL aliquot of an unknown MnO4 sample was placed in a 100.0 mL flask. A 2.00 mL aliquot of a 100.0 ppm MnO4 standard was added to the flask and the absorbance measured to be 0.646. Report your answer to one decimal place. Hint: 1) Remember the dilution factor 2) Yes you have enough information to solve this problem.