Skin cells from the body do not differentiate when they are simply placed in a tissue culture medium; that is, they do not organize into the structure of skin, with different layers and different cell types. What is needed to cause such differentiation to occur? Indicate the most important requirements on any material used.
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- A question from histology. a) Explain the term HE staining b) how many preparations are there for LM? Please answer all parts if possible.A small tumor is excised from a patient's body by a biopsy. A clinical cytopathologist wants to examine the shape, size, and arrangement of the cells within the tumor by routine histology (the study of tissues) by fixing and staining them with hematoxylin and eosin. The best microscopic technique to use would be: O bright-field microscopy O phase contrast microscopy O fluorescence microscopy. O All of the other can be used O DIC microscopyPlease help me identify the cells.
- What is “tissue culture”. What are the steps involved in tissue culture?How do tissue cells look in comparassion to cheek cells using H&E staining?,.a. Explain the purposes of tissue fixation and tissue staining. b. Identify the factors that affect the quality, of tissue fixation and describe the effects of fixation on tissue (e.g., Temperature, size of tissue, time of fixation, or osmolality of fixative.) c. Describe the following types of microscopes and describe reasons for their use. a. Light (compound microscopy), b Polarizing microscopy, c. Phase-contrast, d. Interference microscopy, e. Darkfield, f. Fluorescence, g. Electron (scanning and transmission). h. Confocal microscope. d.…
- You have been asked to do a strucutural study to understand how increasing cell surface area affects cell size, cell shape and functions. What cell type would help you most efficiently observe the effects of increasing surface area? Choices: A. Monocytes from the blood serum. Monocytes are largely roughly spherical cells. B. Red Blood cells from the bone marrow. The shape of Red blood cells is a biconcave disk and looks like a donut. C. Stem cells from skin. In this case assume a skin stem cell is flat and plate like. D. Microvilli covered cells from the intestines. Microvilli are tiny hair like protrusions in the plasma membrane of intestinal cells that increase the absorption of nutrients in the intestines.Some strains of Staphylococcus aureus produce exfoliative toxin, a chemical that causes portions of the entire outer layer of the skin to be sloughed off in a disease called scalded skin syndrome.Given that cells of the outer layer are going to fall off anyway, why is thisdisease dangerous?Which chemical will be used to stain the cheek cells?
- You are a paralyzed patient in need of surgery to help regrow your spinal nerve cells. There are two types of procedures available to you, the use of either adult or embryonic stem cells to regrow your nerve cells. What questions should you ask to identify one difference between adult and embryonic stem cells?What types of stains are used to stain tissues to be viewed with an electron microscope?1.) Draw and label areolar tissue as viewed with a microscope at medium magnification. 2.) Draw and label hyaline cartilage as viewed with a microscope at medium magnification.