Suppose you were in a research lab in Romania and the lab is equipped with several spectrophotometers (UV and VIS). You need to determine the concentration of a protein solution and although you know several techniques you must decide which spectrophotometer to use. Which spectrophotometer are you choosing and which wavelength will you use?
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Suppose you were in a research lab in Romania and the lab is equipped with several spectrophotometers (UV and VIS). You need to determine the concentration of a protein solution and although you know several techniques you must decide which spectrophotometer to use. Which spectrophotometer are you choosing and which wavelength will you use?
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- Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. STD CURVE BEN Amax Ben Amax Jerry STD CURVE JERRY Amax Ben Amax Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287…Which molecules, or function groups, or complexes that may be responsible for the absorbance at 595 and 280 wavelengths? The instrument used is spectrophotometry Determination of protein concentration ( Bradford-Spectrophotometry) Lab
- Which of the following statements are true about the use of a blank in spectrophotometry? Select all that apply. Select 2 correct answer(s) A blank is needed because the spectrophotometer also measures the absorbance of the cuvette and water. A blank must be measured prior to measuring your sample. None of the answer choices listed are correct. A blank must be measured after measuring your sample. A blank is only needed when the concentration of your sample is known.Can spectrophotometry be used to determine the concentration of “colorless” solutes such as salt or sugar? Explain your answer.the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.
- If you place a cuvette with an unknown concentration of that solute in the spectrophotometer and it reads an absorbance of 0.15 AU estimate the concentration of solute insideYou have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).A chemistry student at GGC synthesized a new sulfonamide (S7) drug to help treat malaria. Using a spectrophotometer the student observed that S7 converts to a colorless molecule in the presence of malaria parasite called Plasmodium falciparum. To study the rate of reaction, the student recorded the absorbance/amount of S7 at a wavelength of 570 nm. The results are summarized in the table below: Time (s) Absorbance/Amount of S7 0.80 0.63 10 0.50 15 0.40 20 О.32 25 0.25 30 0.20 Determine the half-life of S7. Include units in your answer MacBook Air 000 000 F8 80 F7