Table 1 - Characteristics of the microscope Low Power (Dry) High Power (Immersion Oil) Features Medium Power (Dry) Focal length (mm) Working distance (mm) Linear Magnification (X) Numerical aperture (N.A.) Diameter of front lens (mm) 16mm 4mm 1.8-2.0mm 4-8mm 0.5-0.7mm 0.1mm 10x 40-45x 90-100x 0.25 0.55-0.65 1.25-1.4 2.0mm 0.4mm 0.2mm
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- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…The objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.
- Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.For a microscope that has an objective lens magnification of 100X and an ocular lens magnification of 5X, calculate the total magnification. 500X O 1000X O 50X O 100X O No listed answer is correct.Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length um
- 1. In the following table, fill in the missing information in order to calculate the Total Magnifica- tion for each objective lens. Use the information provided in the laboratory exercise to help you. Magnification of Magnification of Objective Lens Total Magnification Objective Lens Ocular Lens Lowest Power 4X Higher Power 10X Greater Power 40X Highest Power 100X 2. What would the total magnification be if a microscope had 15X ocular lenses and 4X, 10x, 45X and 95X objective lenses? Insert the correct information in the following table. Magnification of Objective Lens Magnification of Objective Lens Total Magnification Ocular Lens 4X Objective lens 10X Objective lens 45X Objective lens 95X Objective LensCalculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umPART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
- The compound (bright-field) microscope is parfocal. It means that: Question 4 options: objective lenses are built into revolving nosepiece its lenses effectively decrease focal lenght can achieve maximum resolution lower than 0.2 μm image should remain in focus when objective lens is changedComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)Determine the total magnification of a microscope when using the 4X objective lens. (Showyour work/math)