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The absorbance of an iron thiocyanate solution containing 0.00500 mg Fe/mL was reported as 0.4900 at 540nm. Calculate the specific absorptivity including units of iron thiocyanate on the assumption that a 0.100 cm cuvette was used.
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- The absorbance of an iron (III) thiocyanate, Fe(SCN)3 (MM= 230.09 g/mol), solution containing 0.00500 mg/mL is reported as 0.4900 at 540 nm. Calculate the specific absorptivity, including units, of iron (III) thyocyanate on the assumption that a 1.00 cm cuvette is used.A dilute peroxide solution was prepared by quantitatively diluting 10 mL stock H2O2 (MW = 34.0147) to 250mL using a volumetric flask. 50 mL aliquot of the diluted peroxide solution was titrated using the previously standardized KMnO4 in problem 1. Titration of the sample required 29.00 mL titrant and the blank containing 50 mL 1:5 H2SO4 required 0.75 mL of the standard KMnO4. Calculate the concentration in %w/v of the stock H2O2. (Hint: H2O2 produces O2 under acidic condition). Follow sig. FigWhat is the molar absorptivity, in M-1cm-1, for a 4.00×10-5 M solution placed in a 2.00 cm cuvette that is measured to have an absorbance of 0.263?
- The accuracy of a spectrophotometer can be evaluated by preparing a solution of 60.06-ppm K2Cr2O7 in 0.0050 M H2SO4 and measuring its absorbance at a wavelength of 350 nm using a cell with a pathlength of 1.00 cm. The absorbance should be 0.640. What is the molar absorptivity of K2Cr2O7 at this wavelength?The absorbance of an iron(III) thiocyanate, Fe(SCN)3 (MM = 230.09 g/mol), solution containing 0.00181 mg/mL is reported as 0.833 at 540 nm. Calculate the specific absortivity of iron(III) thyocyanate with units cm-1 (mg/mL)-1 . A 0.77 cm sample cuvette is used. Report your answer to 3 significant figures.1) The ratio strength of a 10mL vial of neostigmine methylsulfate injection USP is 1:1500. Calculate the amount of neostigmine methylsulfate in milligrams contained in the 10mL vial. (Round to the whole number).
- A toluene solution (1.50 mg / L) shows an absorbance of 0.30 in a cuvette with a 0.025 mm optical path. Calculate the limit of detection (in ppb) for toluene if the noise with the solvent spectrum is 0.002 absorbance units in a 0.050 mm cuvette.1. There is a substance in a solution (5.00 x10 M). when using a spectrophotometer, you find that the length of cuvette is 2 cm and only 50 % of the certain light beam is transmitted. Calculate the molar absorptivity, including units? and What will be T% if the solution is diluted to twice its original volume? Use the editor to formmat your answerThe concentration of butanol 1.5M. Calculate the molar absorptivity ? when the solution produced an absorbance of 3.2 when using a spectrophotometer with a cuvette path length of l = 1.0 cm
- In a reverse-phase separation, a solute was found to have a retention time of 19.6 min, while an unretained peak had a retention time of 1.45 min when the mobile phase was 20/80 v/v% methanol/water. (b) Calculate the k’ for the solute. (c) Calculate the new k’ if the mobile phase is changed to 60/40 methanol/water assuming identical void volume. (d) Calculate a solvent composition that would bring k’ from initial value to a value of 1.20 using tetrahydrofuran/water mobile phase rather than 20/80 v/v % methanol/water.A solution containing 4.48 ppm KMnO4 exhibits 85.9 %T in a 1.00-cm cell at 520 nm. Calculate the molar absorptivity of KMnO4 at this wavelength.Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.