The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Question
![The BSA stock solution from the previous problem was then diluted to generate a set of standard
solutions of known concentrations. After performing biuret assay on these solutions, their absorbance
at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained.
Concetration of BSA
Absorbance
(mg/mL)
0.1
0.048
0.2
0.095
0.4
0.191
0.6
0.290
0.8
0.380
1.0
0.485
Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an
unknown sample having an absorbance of 0.325.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F32349c60-da4b-4b72-aa44-568f7999fef7%2F2fa57baf-4045-419b-8599-8f67b127d805%2Fgu3epn_processed.png&w=3840&q=75)
Transcribed Image Text:The BSA stock solution from the previous problem was then diluted to generate a set of standard
solutions of known concentrations. After performing biuret assay on these solutions, their absorbance
at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained.
Concetration of BSA
Absorbance
(mg/mL)
0.1
0.048
0.2
0.095
0.4
0.191
0.6
0.290
0.8
0.380
1.0
0.485
Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an
unknown sample having an absorbance of 0.325.
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