The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes. From the graph try to estimate retention times for peaks 2 and 3. Retention time peak 2 = Retention time peak 3 = Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3. Assume that tm is 2.00 minutes. How much time does gamma-tocopherol spend in the stationary phase? From tr and w1/2 find N for the peak that elutes first. From tr and w1/2 find the resolution of peaks 2 and 3 Calculate α for these peaks. If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes. From the graph try to estimate retention times for peaks 2 and 3. Retention time peak 2 = Retention time peak 3 = Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3. Assume that tm is 2.00 minutes. How much time does gamma-tocopherol spend in the stationary phase? From tr and w1/2 find N for the peak that elutes first. From tr and w1/2 find the resolution of peaks 2 and 3 Calculate α for these peaks. If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
Chemistry & Chemical Reactivity
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Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
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The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes.
From the graph try to estimate retention times for peaks 2 and 3.
Retention time peak 2 =
Retention time peak 3 =
Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3.
Assume that tm is 2.00 minutes.
How much time does gamma-tocopherol spend in the stationary phase?
From tr and w1/2 find N for the peak that elutes first.
From tr and w1/2 find the resolution of peaks 2 and 3
Calculate α for these peaks.
If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
Transcribed Image Text:1.
alpha-Tocopherol
2. Menadione
3. gamma-Tocopherol
4. Chlolecalciferol
0
ساله
2
4
2
6
Min
8
4
10
12
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