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The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. [catechol] (mM) Rate (AAU/min) Rate (AAU/min) Uninhibited PBHA 1,01 65,02 38,27 0,49 47,11 24,60 0,32 38,44 16,99 0,25 32,84 13,88

The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. [catechol] (mM) Rate (AAU/min) Rate (AAU/min) Uninhibited PBHA 1,01 65,02 38,27 0,49 47,11 24,60 0,32 38,44 16,99 0,25 32,84 13,88

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. 1,01 0,49 0,32 0,25 0,19 [catechol] (mM) Rate (AAU/min) Uninhibited 65,02 47,11 38,44 32,84 26,92 Rate (AAU/min) PBHA 38,27 24,60 16,99 13,88 11,13 Using graphical methods, determine the uninhibited reaction's Km and Vmax. Also determine whether PBHA is an inhibitor of o-diphenyl oxidase. If it is, specify which type of inhibitor it is and motivate your answer. Gebruik grafiese metodes en bepaal die ongeïnhibeerde reaksie se Km en Vmax. Bepaal ook of PBHA 'n 'inhibeerder van o-difenieloksidase is. Indien wel, spesifiseer watter tipe inhibeerder dit is en motiveer jou antwoord.
Transcribed Image Text:The following data is for the oxidation of catechol (the substrate) to o-quinone by the enzyme o-diphenyl oxidase. Additional data is also provided for when the reaction is run in the presence of p-hydroxybenzoic acid (PBHA). All data was acquired by monitoring the change in absorbance at 540 nm. Die volgende data is vir die oksidasie van katekol (die substraat) na o-kinoon deur die ensiem o- difenyloksidase. Bykomende data word ook verskaf vir wanneer die reaksie uitgevoer word in die teenwoordigheid van p-hidroksibensoësuur (PBHA). Alle data is verkry deur die verandering in absorpsie by 540 nm te monitor. 1,01 0,49 0,32 0,25 0,19 [catechol] (mM) Rate (AAU/min) Uninhibited 65,02 47,11 38,44 32,84 26,92 Rate (AAU/min) PBHA 38,27 24,60 16,99 13,88 11,13 Using graphical methods, determine the uninhibited reaction's Km and Vmax. Also determine whether PBHA is an inhibitor of o-diphenyl oxidase. If it is, specify which type of inhibitor it is and motivate your answer. Gebruik grafiese metodes en bepaal die ongeïnhibeerde reaksie se Km en Vmax. Bepaal ook of PBHA 'n 'inhibeerder van o-difenieloksidase is. Indien wel, spesifiseer watter tipe inhibeerder dit is en motiveer jou antwoord.
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