The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235
The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter13: The Chemistry Of Solutes And Solutions
Section: Chapter Questions
Problem 42QRT
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![The protein content of the unknown samples were determined using BSA as standard. The
absorbance values of the standard solutions and samples are as follows.
BSA concentration (mg/mL)
Absorbance
0.000
20
0.110
40
0.192
60
0.248
80
0.325
100
0.400
120
0.460
a. What is the equation of the line?
b. Determine the protein concentration of samples in ug/mL.
Sample dilutions
1 in 14
A540
0.150
1 in 5
1 in 8
0.380
0.235](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F32349c60-da4b-4b72-aa44-568f7999fef7%2F551fcf8e-6a06-4947-b9bd-e55727e3a54a%2Fi9rvzzp_processed.png&w=3840&q=75)
Transcribed Image Text:The protein content of the unknown samples were determined using BSA as standard. The
absorbance values of the standard solutions and samples are as follows.
BSA concentration (mg/mL)
Absorbance
0.000
20
0.110
40
0.192
60
0.248
80
0.325
100
0.400
120
0.460
a. What is the equation of the line?
b. Determine the protein concentration of samples in ug/mL.
Sample dilutions
1 in 14
A540
0.150
1 in 5
1 in 8
0.380
0.235
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