The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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5. The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data?

The activity of the GAPDH
enzyme was assayed in the
presence of a constant
amount of glyceraldehyde-
3-phosphate and an
increasing amount of
NAD. The activity of the
control (that is, the enzyme
with no other metabolites)
was compared to the
activity of the enzyme in
the presence of various
metabolites. The results are
shown in the figure to the
right.
Units/mg protein
R
8
9
10
-NADP+
Hill coeficient = 1
Hill coefficient = 2
NAD], mM
15
Control -- AMP-X-Glucose-1-P
20
Transcribed Image Text:The activity of the GAPDH enzyme was assayed in the presence of a constant amount of glyceraldehyde- 3-phosphate and an increasing amount of NAD. The activity of the control (that is, the enzyme with no other metabolites) was compared to the activity of the enzyme in the presence of various metabolites. The results are shown in the figure to the right. Units/mg protein R 8 9 10 -NADP+ Hill coeficient = 1 Hill coefficient = 2 NAD], mM 15 Control -- AMP-X-Glucose-1-P 20
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wouldn't ADP, a reactant of the other (phosphorylating) pathway, tend to inhibit the competing non-phosphorylating pathway (in order to get more ATP made)? And the reverse for ATP (whose presence indicates that pathway may not be needed any more)?

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Follow-up Question

NADP+ and control are switched in the Km values (graph is not clear on this), would this make NADP+ an inhibitor?

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