TRUE OR FALSE: 1. The 35S precursor is the precursor RNA transcript for all ribosomal RNAs except the 5S RNA.
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TRUE OR FALSE:
1. The 35S precursor is the precursor RNA transcript for all ribosomal RNAs except the 5S RNA.
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- www D le C 3⁰ A B Indicate True (T) or False (F) for the following statements. Only use the letter (T/F) in the space provided 1. The name of this process is best known as Rho dependent termination 2. The enzyme C called DNA polymerase incorporates ribonucleotides into B called the mRNA False 3. The DNA region A contains inverted palindrome sequences which results in formation of a stem-loops structure 4. During this process, the structure D called terminating hairpin forms and increases the enzyme affinity which terminates transcriptionTrue or False: 1. Amino acid chain elongates when the AA from the peptidyl binding site is added to the AA of the tRNA-aa complex of the aminoacyl binding site. 2. Priming for an mRNA transcript is done by an RNA polymerase.Match the correct answer to each blank. Note that each answer (1 through 4) can be used more than once Eukaryotic mRNAs Prokaryotic mRNAs Transfer RNAs Ribosomal RNAs 1. They are capped on their 5’ ends and polyadenylated on their 3’ ends 2. They contain unusual or modified nitrogenous bases 3. There are 4 types of these in eukaryotes and only 3 types in E. coli 4. They contain introns
- 41. The following 9 TRNAS have anticodon loop regions that base pair with the mRNA in the ribosome. Using your knowledge of tRNA-MRNA base pairing and the genetic code chart (+lecture slides), indicate the MRNA codon message being read by the ribosome as well as the1-letter amino acids that is generated. Anticodon Anticodon Anticodon Anticodon 4 Anticodon Anticodon 6. 2 5'-IAG-3' 5'-UAU-3' 5'-АAА-3' 5'-CUC-3' 5'-GAU-3' 5'-AGA-3' codon MRNA 5'-__-3' 5' 3' 5' 3' 5' 3' 5' 3' 5' 3' 1 letter amino acid Anticodon Anticodon Anticodon 8 7 5'-CCG-3' 5'-GUU-3' 5'-CGC-3' codon 5'-__-3' 5' 3' 5' _3' mRNA 1 letter amino acidAn unprocessed pre-mRNA has the following structure. Which of the following is not a possible size (in bp) of the mature mRNA? 205bp 180bp 150bp 100bpAbout 95% of the average human transcription unit consists of: a. short repeat sequences. b. protein-coding sequences. c. regulatory sequences. d. introns. e. origins of replication.
- Choose the correct option for following three mcqs 16.Termination codons differ from other codons in that: They only recognize a single tRNA They are capable of binding to many different tRNAs They bind to release factors instead of tRNAs They only specify a single amino acid Termination codons do not differ from other codons 17.Which of the following statement/s about the wobble hypothesis is/are TRUE? An mRNA codon may pair with more than one transfer RNA Several mRNA codons may pair with a single transfer RNA There are too many tRNAs present to account for the number of amino acids Most mRNA codons can pair with only a single transfer RNA tRNAs wobble when attached to an mRNA 18.Which of the following statements about protein synthesis is FALSE? It begins with methionine It usually ends at UAA, UAG or UGA During translocation small subunit of ribosome moves first It is from N terminus to C terminus In bacteria it begins while mRNA is being synthesizedput in correct order the steps for initiating translation. 1. Binding of initiator tRNA to mRNA 2. Binding of large ribosomal subunit to mRNA 3. Binding of small ribosomal subunit to mRNA 4. Binding of tRNA with 2nd amino acid to the A site 5. Formation of covalent bond between methionine and second amino acid A) 1, 2, 3, 4, 5 B) 3, 1, 2, 4, 5 C) 1, 3, 2, 4, 5 D) 2, 3, 1, 4, 5 E) 3, 2, 1, 4, 52. A single base addition and a single base deletion approximately 15 bases apart in the MRNA specifying the protein lysozyme for the bacterial virus T4 caused a change in the protein from its normal composition: Lys – Ser - Pro - Ser - Leu- Asn-Ala - Ala - Lys To the mutant form: Lys - Val - His- His - Leu - Met- Ala-Ala- Lys a. From the genetic code, decipher the sequence of mRNA for both original protein and double mutant. b. Which base was added? Which base was deleted? · END -
- 6. Indicate whether each of the following events occurs when tryptophan is high or when tryptophan is low by placing a check in each of the appropriate blanks. Share your reasoning. Tryptophan Tryp high Event Ribosome does not stall at Trp codons Region 2 of the leader pairs with region 3 Ribosome covers part of region 2 of leader Transcription is terminated before structural genes are transcribedSection B: True / False (5 marks) 1. Primase has a DNA dependent RNA polymerase activity. 2. 7-methylguanosine cap is added to the 5' end of the DNA after replication in eukaryotes. 3. snRNA is produced in transcription. 4. Initiator tRNA enter ribosome with the help of EF-Tu. 5. At low oxygen partial pressure Po2, myoglobin has lower oxygen affinity than hemoglobin.Main... My Drive - Google... 8. Which statement/s about RNA is/are true? (Select all that apply.) * RNA is made through the process of translation RNA is made through the process of transcription and translation RNA is made through the process of transcription Some types of RNA just exist in the nucleus without ever needing to be made 9. Identify the TRUE statement about the process of transcription: * RNA polymerase requires an RNA primer molecule before it can begin transcripti DNA is not needed for the process of transcription. RNA polymerase binds to a promoter region in the DNA to initiate transcription. During the elongation phase, RNA transcripts are built from 3' to 5'. 10. Which is correct? * Translation occurs in the nucleus; transcription occurs in the cytoplasm Transcription occurs in the nucleus; translation occurs in the cytoplasm Both transcription and translation take place in the cytoplasm Both transcription and translation occur in the nucleus 1