Tube 1 2 3 4 Dilution or DF 5X 2:3 3/5 4:1 Volume of stock (ml) Volume of distilled water (ml) Absorbance @ 615 nm Diluted solution conc. (mm)

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.19QAP
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1. Indicate the volume of stock solution (2 mM) and distilled water (diluent) required to
prepare 5 mL of each of the dilutions indicated in the table below. Appropriately number
and title this table
Table No. and Title:
Tube
1
2
3
4
Dilution
or DF
5X
2:3
3/5
4:1
Volume of
stock (ml)
Volume of
distilled
water
(mL)
Absorbance @
615 nm
Diluted
solution
conc. (mm)
2. Using the equations provided above, calculate the concentration of each of the four
dilutions (Tubes 1-4); record these values in the table above. Show your calculations for one
of the dilutions (your choice) in the space below.
I
1
I
I
I
I
1
1
I
I
I
I
I
I
I
I
I
1
I
I
I
I
1
I
I
1
I
1
I
I
1
I
I
I
Transcribed Image Text:1. Indicate the volume of stock solution (2 mM) and distilled water (diluent) required to prepare 5 mL of each of the dilutions indicated in the table below. Appropriately number and title this table Table No. and Title: Tube 1 2 3 4 Dilution or DF 5X 2:3 3/5 4:1 Volume of stock (ml) Volume of distilled water (mL) Absorbance @ 615 nm Diluted solution conc. (mm) 2. Using the equations provided above, calculate the concentration of each of the four dilutions (Tubes 1-4); record these values in the table above. Show your calculations for one of the dilutions (your choice) in the space below. I 1 I I I I 1 1 I I I I I I I I I 1 I I I I 1 I I 1 I 1 I I 1 I I I
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