Using a Light Microscopé to PRE-LAB QUESTIONS Fill in the diagram of the microscope with the term or description that matches the microscope part. Eye Plece: Body Tube Contains lens to increase magnification usually 10x Revolves to allow changing various objectives Arm Objectives Moves stage up and down approximately to correct distance Hold slides in place Stage Permits finer focusing by moving the stage in smaller increments Regulates the amount of light going through the stage Base Light Source
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- Lesson Focus Specimens Using the Compound Microscope be seen with the naked eye, we use an the observer, This instrument is called a Te magnity ohjects specially thoe kwo small nshuent thal magalfies so ial it may be seen mkcencope. It is an eavential ool especialty in studlving and observing very tiny objects. Aside h magnillealion ming a micrescope aho provides resolution that is necessary to clearly view speeien. Te property fous a speoimen, you need to follow some steps. These steps shall be diecussed in detail in the prepared activities of this module. What's In Belore we go on with the lesson, it is important to recall your past lesons that are related to in he iR ui nd mim Vam kin wieden nht this leson that you have leaned from the past lesson will be very helpful to better understand our topic. Are you ready now? Let's stact! Match the parts of a compound microscope to its fanction Write your anvwers on a separate paper. 1. This part controls the amount of light parving thugh…What is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-ProbeComparison of the different objectives of the compound microscope Point of Comparison Scanner LPO НРО O1O Degree of magnification (X) 4х 10x 40x 100x Details of the specimen (least to most detailed) Area of field of view (smallest to biggest) Brightness of image (dimmest to brightest ) Working distance (mm)
- Match the terms on the left with its description _____ stage _____ocular lens ____Diaphragm _____adjustment focusing knob _____Body tube holds the microscope slide lens you look through, usually 10x power regulate the amount of light passing the specimen used to focus on specimen holds the eyepiece upper part of the microscope that contains optical components. holds the object lens magnifies specimen secures that slice to the stage condenses light from the light source into a narrow beam source of illumination magnifies an image 100x,Give a particular use/uses of the following types of a microscope: 1. Research microscope 2. Stereomicroscope 3. Student microscope 4. Projection microscopeUsing the provided materials and the microscope itself, review the parts of the microscope including the name, where it is located, its function and the appropriate times to use. Record your observations and notes as vou will require this information when writing yourlab report
- Help withWhat part of the microscope is used to make large adjustments to the height of the stage? coarse focus O eye pieces fine focus slide holder adjustment O iris diaphragmIn order to visualize the fine structure of viruses and cytoskeletal filaments at 10-25 nanometers in diameter the type of microscopy that would be most effective is O standard light microscopy O phase-contrast light microscopy transmission electron microscopy O darkfield light microscopy O differential-interference microscopy
- Artefacts seen in virtual slides of given data below. Microscopy not radiology.Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4Write the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.