What are the basic concepts and precautions to be observed in specimen collection for Microbiological examination? Enumerate specific examples of clinical specimens collected for microbiological examination. Give 2 specific examples of bacteria isolated from clinical samples. What is a transport medium; give specific examples and state their purpose.
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- What are the basic concepts and precautions to be observed in specimen collection for
Microbiological examination? - Enumerate specific examples of clinical specimens collected for microbiological examination. Give 2 specific examples of bacteria isolated from clinical samples.
- What is a transport medium; give specific examples and state their purpose.
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- Describe the purpose of identifying unknown bacterial cultures in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms.Describe the purpose of identifying unknown bacterial culture in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms.Human error is a factor in the Kirby-Bauer procedure that often contributes to variation in zone size. Circle the effect on the zone size if the following occurred: ● ● Overinoculating the agar with bacteria: Waiting too long to place disks after inoculation: Using a culture that is less than 0.5 MacFarland: False increase False increase False increase False decrease False decrease False decrease
- Briefly discuss the following topic and include appropriate examples where necessary. Physiological characteristics employed in microbial classification.Place the descriptions into the correct banners of this infographic to show your understanding of phenotypic diagnostic methods. Study stained specimens under magnification Detect the array of enzymes possessed by the microbe Direct Examination Blochemical Testing Phage typing or growth in animal cells/hosts Does not require cultivation Require cultivation Susceptibility Testing Use specialized media to isolate specimen and complete macroscopic observation Determine what antimicrobials are effective against the microbe Selective/ Differential Growth MiscellaneousGive the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. Vials
- Please match the fields of microbiology with the statements that most accurately describe them to test your understanding of the primary areas of study within microbiology. 1. The field dedicated to monitoring and controlling the spread of disease within a population (Click to select) 2. The field which uses microbes to produce specific desired products (Click to select) 3. The field which studies the protective reactions to microbial infections, examples include blood testing and vaccination (Click to select) 4. The field which manipulates DNA of an organism to create a new organism with a desired trait (Click to select) 5. The field which studies the relationships between microbes and domestic plants and animals (Click to select) 6. The field which is concerned with food-borne diseases as well as food and beverage production (Click to select)困 Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following is/are TRUE the use of laboratory instruments and regarding the use laboratory instruments equipment in regards to making of culture media? " and equipment? * Air bubbles on the surface of agar plates Hot plates can be used to sterilize the inoculating loop and needle. can be removed by fanning them with the luminous flame of the bunsen burner. If the agar surface is wet, it can be dried by A drying oven is used to sterilize nutrient agar, a solid culture medium. heating at 30-40 deg C in the drying oven before inoculation is done. The refrigerator is used to grow psychrophiles. All media taken from the refrigerator should be warmed to room temperature before use, P Type here to search 7:11 pm 30/09/2021 ヘロG) ENG 近MALDI-TOF, is a method for identifying bacteria quickly. Look at this graphic then put the steps in order. Please follow a pathway of steps involving the Bacterial Culture: Get a readout of species identification and (sometimes) whether it is resistant or susceptible to antibiotics. Use colony from a nutrient agar plate. Prepare the sample, run it through MALDI-TOF machine. Grow a clinical sample on nutrient agar.
- Please match the test with its description to assess your understanding of the kinds of data collected during information gathering and your understanding of the processes involved in identifying microbes from samples. 1. using macroscopic and microscopic traits for identification appearance 2. tests that determine chemical characteristics including enzyme production and nutritional requirements of the microbe biochemical tests 3. analyzing the genotype of an organism DNA profiles 4. tests the organism against known antibodies to determine if there is a reaction between the organism and the antibody immunologic testing vA patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000