What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure. (2675) Есo01091PfoI (46)(2621) AatII(2619) ZralNdel (184)BSTAPI (185)(2503) SspIKasI (235)NarI (236)Sfol (237)PluTI (239)sig...AmpR promoterАрol - EcoRI (396)Eco53kI (404)BanII - SacI (406)Acc65I (408)2500(2298) XmnIAvaI - BSOBIKpnITspMIXmaI (412)laczaBтеT1101 (413)Smal (414)BamHI (417)Xbal (423)SalI (429)АccI (430)HincII (431)PstI - SbfI (439)BfuAI - BspMI (442)SphI (445)HindIII (447)(2179) Scal(2098) TsoIPUC192686 bpBspQISapI (690)-AfIIII - PCİI (806)(1847) NmeAIII(1779) BsrFI(1769) BpmI(1760) BsaIori(1699) AhdI1500BseYI (1110)PspFI (1114)AlwNI (1222)500| lac promoterlac operatorMCSM13AmpR2000

Transcription is the process of synthesis of RNA using DNA as the template and is catalyzed by

Answered: What is the direction of transcription…

What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

100%

What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure.

(2675) Есo01091
PfoI (46)
(2621) AatII
(2619) Zral
Ndel (184)
BSTAPI (185)
(2503) SspI
KasI (235)
NarI (236)
Sfol (237)
PluTI (239)
sig...
AmpR promoter
Арol - EcoRI (396)
Eco53kI (404)
BanII - SacI (406)
Acc65I (408)
2500
(2298) XmnI
AvaI - BSOBI
KpnI
TspMI
XmaI (412)
lacza
BтеT1101 (413)
Smal (414)
BamHI (417)
Xbal (423)
SalI (429)
АccI (430)
HincII (431)
PstI - SbfI (439)
BfuAI - BspMI (442)
SphI (445)
HindIII (447)
(2179) Scal
(2098) TsoI
PUC19
2686 bp
BspQI
SapI (690)
-
AfIIII - PCİI (806)
(1847) NmeAIII
(1779) BsrFI
(1769) BpmI
(1760) BsaI
ori
(1699) AhdI
1500
BseYI (1110)
PspFI (1114)
AlwNI (1222)
500
| lac promoter
lac operator
MCS
M13
AmpR
2000

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