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- 1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?SPECTROPHOTOMETRY 1.) Slope of regression line or Molar absorptivity (µM -1cm-1) is 0.0253 and absorbance of unknown solution is 0.131. What is the concentration of the unknown solution (µM )?The ppm concentration of Pb2+ in a blood sample were measured with Spectrophotometry. 5.00 mL of a blood sample were taken and this sample gave a signal of 0.301 a.u.. Another 5.00 mL of a blood sample were mixed with 0.50 mL og 1.75 ppm Pb2+. Then, this mixture was diluted to 25.00 mL and this diluted mixture gave a signal of 0.406 a.u.. What is the ppm concentration of a blood sample?
- 100 MASS SPECTRUM 80 60 40 20 0.0 10 20 30 40 50 60 m/z NIST Chemistry WebBook (https://webbook.nist.gov/chemistry) 1. What is the M'? 2. What is the m/z of the base peak? 3. Does it contain chlorine? Explain your answer. Rel. Intensityuse the following chromatogram and table as your data. VWD: Sgrad A 284 nm Retention Time 40- -40 20 20 5 10 15 20 25 30 35 Minuteo VWD: Signal A, 284 nm Retention Area Identity Time (min) 7.867 3214697 Vitamin A 8.493 15687 19.410 140354 19.710 64346 20.803 884315 Vitamin E 21.640 36745 22.100 63054 8.403 L98 ÞI 20.803 ; 84年 81A solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is: Blue dye stock solution = 0.293 M Absorbance at 630 nm = 0.00265 Calibration curve y = 0.0833x A.) What is the theoretical molar concentration? B.) What is the experimental molar concentration? C.) What is the percent error?
- 29. What exactly is relative retention and retention time? How do you solve problems like this?3. The lead in a swab sample, lead standards, together with a blank were made up in 5.00 mL volumetric flasks using 0.2 % HNO3. 20 μL aliquots of these solutions were injected into the spectrophotometer and the absorbance measured at 217 nm. The following results were obtained. lead / ppb Absorbance 0 0.0591 10.00 0.0858 50.00 0.1926 100.0 0.3260 150.0 0.4594 200.0 0.5929 Swab sample 0.3700 (a) Determine the amount of lead in the swab sample in μg.A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc, was found to be 209.6 s with a baseline peak width, w., of 14.4 s. The retention time for aspartame, ta, was 260.0 s with a baseline peak width, wa, of 21.2 s. The retention time for the unretained solvent methanol was 49.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = um %3D Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2. R2N =
- CPU/RAM ProctorU e Proctor e Proctoru o.com/collab/ui/session/join/43918c0bf9cb421ca7b98b0075f5f239 Firefox Ex SPECTROSCOPY 1. If the concentration of solute in a solution is high, the Absorbance will be high or low and why? 2. Was the spectroscopy experiment qualitative or quantitative analysis? 3. If we have 12M HCI and we need 3 L of 1M HCI, how much 12 M should we use? 4. What does Molarity mean? 5. When must safety goggles be worn? 6. If you make a mistake recording data, what must you do? spec hw.pptx (2/3) ..% AnA student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μmA chromatogram of a mixture of species A, B, and C provided the following data: a) Define "Retention time". b) Calculate the resolution between species A and B. c) Calculate the retention time of species B necessary to separate it from species A with a resolution of 1.5.
