Q: Is staphylococcus epidermidis a halophile?
A: Bacteria grows when they meet the certain requirement like- chemical, physical conditions in their…
Q: Why is it important for the commercial canning process to destroy the endospores of Clostridium…
A: An endospore is basically a dormant, tough and non-reproductive structure produced by some bacteria.
Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: Which characteristic of the species used determines whether sterility was achieved when autoclaved?
A: Microorganism: These are too small organism which is impossible to see by naked eyes. They exist in…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Why does motility media contain a lower amount of agar than ordinary agar?
A: Agar is a phyco-colloid polymer of galactose monomers obtained from red algae (Gelidium and…
Q: What are the purposes of the antimicrobial agents present in the selectivemedia used for the…
A: the purposes of the antimicrobial agents present in the selective media used for the isolation of…
Q: Why del factor is important in media sterilization?
A: Sterilization, which was called by various means such as Del factor, Nabla factor and the…
Q: What is the purpose of the mashing step in beer-making?
A: Beer is an alcoholic beverage. The production of beer involves the following steps : malting,…
Q: what type of bacteria would be used to make sauerkraut?
A: Sauerkraut is product of cutting raw cabbage that fermented by various lactic acid bacteria. The…
Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…
A: Given data: Each plate contains- LB agar=20ml Ampicillin= 150 micrograms per ml. Amount of…
Q: Why are complex media preferable to chemically defined media for routinecultivation of…
A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…
Q: Why is the time of exposure and temperature required for sterilization greater for dry heat than for…
A: Any technique that removes, destroys, or deactivates all forms of life and other biological agents…
Q: Why is it important to determine the number of mesophilic spores in a food ingredient?
A: Sometimes spore forming mesophilic bacteria found in food ingradient, mainly in dairy product. It…
Q: What bacteria grow on both a MacConkey agar plate and Mannitol Salt agar?
A: Selective media The media is a kind of growth media which only allow the growth of a perticular…
Q: If yellow xanthophylls were present in the extract, why did the extract appear green?
A: Introduction: Xanthophylls are a class of oxygen-containing carotenoid pigments that is liable for…
Q: In the differential absorption heterophile test, what findings are diagnostic of IM?
A: Infectious mononucleosis is a very common disease in adults with symptoms such as malaise,…
Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…
A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…
Q: Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of…
A: Sulfanilic acid reacts with nitrous acid to form diazotized sulfanilic acid if introduced. This…
Q: How alcohol is effective at contolling microbial growth?
A: Sterilization: Sterilization includes the complete wipeout of all microscopic life on a surface.…
Q: What advantages do solid media offer for the isolation of microorganisms?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: What is an alternative to autoclaving for sterilizing heat-sensitive materials?
A: Any process that kills, eliminates, deactivates, or removes all forms of microorganisms and…
Q: Why do you think autoclave sterilization of media is preferred over open flame?
A: An autoclave is used to sterilize surgical equipment, laboratory instruments, media and other…
Q: What is the result of Staphylococcus epidermidis in the TSI agar test?
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: What is the purpose of adding sodium bicarbonate to a culture medium?
A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…
Q: what is sterilization or define sterilization?
A: Sterilization is defined as the process where all the living microorganisms, including bacterial…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: What is the point of the pressure in the autoclave sterilization?
A: Autoclaves are the devices which are like pressure cookers and used in laboratories that provide a…
Q: Why do we add powdered zinc to the nitrate reduction test?
A: Bacteria are prokaryotic simple unicellular organisms. Their mode of respiration may be aerobic or…
Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…
A: Lee Agar is used to study the bacteria that can grow on yogurt. It is primarily used to count the…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: Why is egg enrichment not added to BPA prior to sterilization?
A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…
Q: What test would confirm EHEC Escherichia Ecoli?
A: Bacteria are prokaryotes. Some of them are helpful(Rhizobium helps in nitrogen fixation in…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: Why is cobalt chloride paper used in the experiments?
A: The indicators are used in the experiment to detect the distinct observable change. Many types of…
Q: What type of media components cannot be sterilized in an autoclave?
A: Sterilization refers to the complete destruction or elimination of all viable organisms. The process…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What are the factors that influence the efficiency of chemical sterilization?
A: Chemical Sterilization is the process of removal of microorganisms by the use of chemical…
Q: What conditions are necessary to destroy endospores? In what device are these conditions achieved?
A: Endospores are dormant vegetative cells that have a thick outer covering making them resistant to…
Q: What is the morphology and optimum growing conditions of Thermophilic Molds?
A: These are multicellular filamentous fungi.They have thallus which lack true root ,leaves and stem.…
Q: Based on the bacterial cultivation, what are the possible organisms under its gram reaction?
A: Gram reaction in microbiology is a staining procedure that is used to differentiate bacteria based…
Q: Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental…
A: Chlorination is a disinfection procedure in which microorganisms are killed by the use of chlorine…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
What might have been some of the culture conditions used to isolate A. fumarolicum?
A.fumarolicum is an Extremophile.
They are acidophilic, thermophilic and methanotropic in nature that means they require acidic conditions,high temperatures and methane or methanol for growth and survival.
These microbes lives in vents and fumaroles that eject chemicals,hot nude, fumes, methane gas,etc.
These microbes can grow in extreme conditions such as temperature about 130 degree Fahrenheit, PH as low as 0.8, anerobic conditions,muddy environment.
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Solved in 2 steps
- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaIf no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?
- What is a pure culture?What would happen if the enrichment method in the isolation of bacteriophage was omitted?the chloroform was not added to the enrichment?the 0.1 ml lysate - E.coli mix was plated directly on top of the bottom agar?You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?
- What is a mixed culture? A contaminated culture?One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar plate gave rise to 229 distinct colonies. What was the concentration of bacteria in the original, undiluted suspension?How can the enrichment culture technique be used medically?