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What solution goes into the cuvette when you’re zeroing the spectrophotometer?
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- What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
- What is the diameter of field of view for the high-power objective in millimeters and micrometers?In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.
- If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying power of the objective being used?when you want to compute for the magnification of the specimen, what will you do with the eyepiece and the given objectives magnificationWhat is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens? O 45x O 100x 4.5x O 450x O 10x
- If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400xif the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.