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BIOC 384
The Chemiosmotic Theory
Q11.2: What was the biochemical reason for the "Ox Phos War," i.e., why was Peter Mitchell's hypothesis considered heretical?
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- PTP1B Substrate kcat Km kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEPYLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 ± 0.22 0.53 + 0.02 34.7 + 0.25 52.7 ± 0.7 0.066 + 0.001 (d) (. ) The units for kcat/KM in the above are given according to standard scientific notation. On this basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?Question:- briefly describe the three key elements (number them 1-3) that constitute the mechanism by which oxidative phosphorylation occurs; this is also known as the chemiosmotic cycle or the chemiosmotic theory.PTP1B Substrate kcat Km. kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEP YLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 + 0.22 0.53 + 0.02 34.7 + 0.25 52.7 + 0.7 0.066 + 0.001 ) The units for kcat/KM in the above are given according to standard scientific notation. On this (d) ( basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?
- CHEM151/251 Biochemistry 1 a. Determine Km 1. The following data were obtained for a competitive inhibition study in which the [I] = 3 µM for each determination of vo in the presence of inhibitor. The Vmax = 200 μM P/min for both data sets. 200 Vo (UM P/min) 8 8 8 8 8 8 8 8 8 180 160 140 120 100 40 Name (Print)/ID #: the absence of inhibitor. Participation Question # 10 No Inhibitor 50 +Inhibitor 100 [Substrate] (UM) 150 b. Determine Km, app for the data obtained in presence of inhibitor. 200 c. Calculate the value for Ki. Note: a = 1 + [I]/Ki and a¹ = 1 + [I]/Ki*. for the data obtained inTOPIC: Production of Glutamic acid Pathway -Fermentative production of poly (γ-glutamic acid) from renewable carbon source and downstream purification through a continuous membrane-integrated hybrid process. Conditions: Batch, Aerobic Microorganisms: Corynebacterium glutamicum (NCIM 2168) Inoculum: 10%v/v NCIM-2168 Chemical equation: 2 C6H12O6 + 2 NH3 + O2→ C5H9NO4 + C5H7NO2+ 2CO2+ 7H2O Question: Please explain clearly on the upstream and downstream process that involve in this pathway.25) In biomaterials design enginnering the surface properties effect the biological interactions (TRUE/FALSE) Chemotaxis Production of ECM Migration of the cells Composition of ECM Responsiveness to extracellular signaling 26) The principal functions of the ECM are ; EXCEPT a) Mechanical support for cell anchorage b) Cell proliferation c) Regulates and determine cells dynamic behaviour: d) Scaffolding for orderly tissue renewal
- 1:04 O • + Screenshot (11... / Checkpoint Which of the following are redox reactions? A. NH3 + HCI → NH,CI B. SO3 + H,O → H,SO4 C. Fe,03(s) + CO(g) → Fe(s) + CO2(g) For C... "What is reduced? What is oxidized?"We considered the effects of many parameters in designing bioreactors. Please answer the following questions regarding bubbles in airlift fermenters. Bulleted lists are okay. (a) Why was it undesirable to use a very large bubble size (Diameter >7 mm)? (b) Why was it undesirable to use a very small bubble size (Diameter <7 mm)? (c) Other than varying bubble size, what can be done to further decrease cell death due to entrainment?pls explainWhy was absorbance measured at 420 nm in the enzyme kinetics experiment? To monitor thea. decrease in catechol concentrationb. increase in quinone concentrationc. decrease in polyphenol oxidase concentrationd. polyphenol oxidase-catechol complex concentrationLineweaver-Burk plot is the most used linearization method of the MichaelisMenten equation, but the main drawback is a/an:a. overemphasis to low substrate concentration and less emphasis to high substrate concentration.b. overemphasis to high substrate concentration and less emphasis to low substrate concentration.c. exaggeration to both ends of the reciprocal of the substrate concentration.d. lack of independent variables to x- and y- coordinates
- several days. 20. The apparatus below were used to investigate whether yeast in glucose solution produce carbon(IV) oxide in the absence of oxygen. (a) Ide rep (i) (ii) (b) Sta pat 22. The up rele Oil film flas Lime water dur yeast + 10% Glucose solution (a) Explain how you could remove dissolved oxygen from the 10% glucose solution before the experiment commences. (b) State what would happen to the lime water as the experiment proceeds to the end. (c) Explain what would happen if the temperature of glucose solution with yeast raised from room temperature to 37°C. Flask A a)Give oil b) Stat this ezuation The mrocess represented.BIOC 384 Redox Reactions and Reduction Potentials Q10.1: Answer the following three-part question. a) Calculate the ΔEº’ for the citrate cycle reaction converting malate to oxaloacetate and describe how you calculated this value. b) What is the overall change in standard free energy (ΔGº') for this reaction and describe how you calculated it. c) Do you think the actual change in free energy (ΔG) for this reaction differs significantly from the standard free energy (ΔGº') for the reaction, and if so, why is it different?9:03 3G 1 Search a myatu.net ACCRA MICI U TECHNICAL UNIVERSITY Topic 5 O Bacteriology Not Submitted Due 5 March 2021 1. State the principle that underlies the following biochemical tests: a) Methyl red test b) Voges-Proskauer test how does phosphorylation differ from substrate-level phosphorylation? Topic 6 Topic 7