When it comes to simple stains, why is time so important? And what is the purpose of rinsing the slide in water after each Gram staining procedure?
Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: What does it mean when a microscope is said to be parfocal?
A: Microscope is an instrument which is used to magnify and resolve an image produce by an object which…
Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…
A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: In the differential staining method, various stains are applied. These methods can be utilized for…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why can’t nitric acid stains be removed by washing?
A: Nitric acid: it has the chemical formula HNO3. It is a conjugate acid of nitrate and a protic…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: During studying a thick heavily stained specimen under the microscope, it is better to light…
A: Microscopy is the technique of visualisation of microscopic specimens by using a microscope after…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: Why don't you heatfix a negative stain slide?
A: Negative staining is used to investigate the physical structure, size, and organization of…
Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: What is the advantage of gram staining over simple staining
A:
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: What is acid fast staining and why do we use gram staining more?
A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: do we need to stain microorganisms?
A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
When it comes to simple stains, why is time so important? And what is the purpose of rinsing the slide in water after each
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?What do you think there are 2 staining reagents applied in differential staining?Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each component work? (ie. What does the mordant do?)
- Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?
- Why are basic dyes more effective for bacterial staining than acidic dyes?What do you understand from negative stain, simple stain, and acid-fast stain?Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantWhy are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.what are the positive and negative results of the negative stain procedure?