when testing unpasteurized milk you get a colony count of 118 colonies on plate A and 110 colonies on plate B after you plated .1 mL of a sample your .1 mL sample came from tube #6 you have prepared a serial dilution with six test tubes all tubes are a 1 to 10 dilution calculate the average CFU/ml of the original sample

when testing unpasteurized milk you get a colony count of 118 colonies on plate A and 110 colonies on plate B after you plated .1 mL of a sample your .1 mL sample came from tube #6 you have prepared a serial dilution with six test tubes all tubes are a 1 to 10 dilution calculate the average CFU/ml of the original sample

Complete Textbook Of Phlebotomy

5th Edition

ISBN:9781337464314

Author:Hoeltke

Publisher:Hoeltke

Chapter10: Sample Preparation And Handling

Section: Chapter Questions

Problem 4RQ

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Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uL
First There Was One, Then There Were Two RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each Dilution Plated Time Sampled 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10:40 TNTC TNTC TNTC 280 TFTC TFTC3 T FIC2 TFTC TFTC 13 TNTC TNTU TNTC TNTC I60 TNTC TNTC 1:00 TFTC q a TFTC O 1:20 11:40 12:00 TNTC TNTC87 TFTC TFTC, TFICg TNTC ITNTC ITNTC TNTC 125 TNTC TNTC TNTC TNTC 164 TFIC 13 T+TC Calculate the cfu/ml for each time sample you worked with in the space below and record those values in the table below.
1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes
Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
Which of the following is FALSE about the Nitrate Reductaion Test, procedure and interpretation? Question 2 options: A Nitrate medium contains 0.1% potassium nitrate as a substrate B Reagent A and B are used to determine the presence of nitrite C When zinc is added, it will react with any nitrate and turn red D A tube with red after zinc is a positive test for nitrate reduction E A tube that turns red after reagent A and B is a positive test
1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______
1mL Stock #1 1mL 9mL #2 9mL #3 1mL A 9mL #4 0.1mL 1mL O O B 9mL #5 1mL 1mL 9mL #6 O Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units. 0.1mL 1mk D O
1. a 1 mL stock sample is added to 9 mL Of diluent to make solution A, and then 2 mL of solution A is added to 18 mL of diluent to make solution B. what is the final dilution with respect to the stock? 2. in most probably number (MPN) testing what media is used for confirming test and what does a positive result looks like? 3. In Most probably number (MPN) testing what can be inferred from a positive result on the presumtive test?
3. You were instructed to add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL of sterile diluent. Instead, you add all 5.0 mL to the 125.25 mL, What was the intended dilution and what was the actual dilution? ited States) E Focus OCT 30 W MacBook P DII DD F7 F8 F9 F10 F4 F5 F6 F3 2$ 4 081 R F G H. K < CO
1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred 0.1 mL from the fecal sample 0.1 ml I ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL 0.1 ml A C saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 9.9 ml 9 ml 9.99 ml saline saline saline D 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
A. Experimental Results Sample No. 1 2 3 4 5 1 Sample No. Sample Identity 2 3 + Table 1. Results of the Qualitative Tests of Unknown Lipid Samples Copper acetate Test Greenish-blue color Colorless Colorless Greenish-blue color Colorless 5 Acrolein Test Hubl's Test Ammonium Molybdate Test No precipitates No precipitates No precipitates No precipitates Yellow precipitates No distinct odor Pungent odor No distinct odor No distinct odor Pungent odor Brown solution Light pink color Brown solution Light pink color Light pink color Table 2. Sample Identity of Unknown Lipid Samples Rationale Liebermann- Burchard Test No color change No color change Bluish-green color No color change No color change