Analyse the data proved. Which of the E. coli cultures from the regrowth experiment should be used in mutagenesis experiment. Why you selected and why you rejected？ Table 2. The number of cells per milliliter in the overnight cultures prior to dilution in theregrowth experiment.<R1 cells/ml R2 cells/ml R3 cells/ml R4 cells/ml R5 cells/ml R6 cells/mlE. colicultureAB6666400056000000 6320000068800000 6906640072800000 117866400 63200000664000006480000078666400 78666400562664007600000070400000728000007120000068000000 Growth (OD 600)2.62.42.2Bacterial Growth Curve21.81.61.41.210.80.60.40.200 1 2 3 4 5 6 7 8 9TII I I IIIIIIIII+JIITIII IIHHAII IIABCHHHLRAHITT TTTT图表区H-HHHOIHHHHHHHOHHRHHHHHI IHHHHHHHHH10 11 12 13 14 15 16 17 18 19 20Time (hours)HIHII21 22E

Mutagenesis is the phenomenon in which a change in the original sequence of DNA takes place…

Answered: Which of the E. coli cultures from the…

Science

Biology

Which of the E. coli cultures from the regrowth experiment should be used in mutagenesis experiment

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Q: One of the pathways required for neural tube closure is the WNT signaling pathway. The WNT receptor…

A: The WNT signaling pathway is a complex and evolutionarily conserved cellular communication system…

Q: The following features is(are) the requirement(s) for homologous recombination to occur: A) Very…

A: In living organisms, homologous recombination is a critical mechanism for maintaining genomic…

Q: What is known about the genus? Who discovered this species? When and where was your species first…

A: Polyommatus is a genus of butterflies in the family Lycaenidae. It is found in the mountains of…

Q: The information collected in St. Paul can be used to calculate the storage rate for each watershed,…

A: Storage rate = Total flux in - Total flux out. In the above question, the the components of flux-in…

Q: Can you draw a prokaryotic heterotrophic electron transport chain?

A: The process of cellular respiration involves the oxidation of biological fuels in the presence of an…

Q: Write an abstract of the article below in Research objectives (2 sentences): SPECIFIC OBJECTIVES •…

A: The article investigates the potential of using readily-available inocula in developing countries to…

Q: 1. In endospore staining, what is the purpose of using the steam? 2. Would it be useful to perform…

A: The Gram stain is a laboratory method for classifying bacterial species into two groups according to…

Q: Sarin (chemical name: propan-2-yl methylphosphonofluoridate) is considered a weapon of mass…

A: Neostigmine A cholinesterase inhibitor used in the treatment of myasthenia gravis and to reverse the…

Q: Briefly discuss the following topics and include appropriate examples where necessary. Uses of…

A: A family of large, rod-shaped viruses called baculoviruses infects insects, especially the larvae of…

Q: The addition of poly(U) is important in the degradation of mRNAs encoding which of the following…

A: The addition of poly(U) refers to the process of adding a string of uridine (U) nucleotides to the…

Q: 1-Pedigrees exhibiting consanguinity, show increased presence of _________ traits? a-Dominant…

A: Consanguinity refers to the mating or breeding of closely related individuals such as cousins or…

Q: for the organism Polyommatus eleniae Use this link to answer the following:…

A: Polyommatus eleniae, commonly known as the silvery blue butterfly is a species of butterfly in the…

Q: When it comes to controlling microbial growth in microbiology lab, why is autoclaving so vital? What…

A: Microbial growth refers to the increase in the number of microorganisms such as bacteria, fungi,…

Q: Which species causes the waterborne disease giardiasis? euglenoids trypanosomes diplomonads…

A: A waterborne disease is an illness caused by microorganisms that are transmitted through…

Q: What is the purpose of wild type (C.elegans) grown on plates seeded with pos-1 ? What is the…

A: C. elegans (Caenorhabditis elegans) is a nematode or roundworm species that is commonly used as a…

Q: Explain how polyploidy in plants can generate new plant species. Be sure to provide molecular…

A: Polyploidy refers to organisms that have more than two sets of chromosomes in their cells. While…

Q: Describe the main stages in the formation of a blood clot and evaluate the importance of specific…

A: The process of blood clotting, or coagulation, is a vital mechanism for preventing excessive blood…

Q: For this assignment you will be building a concept map of a specific biome’s characteristics and the…

A: A biome is a large geographical region characterised by a specific type of climate, vegetation, and…

Q: The following are reasons why randomized control trials (RCTs) are not used to investigate all…

A: RCTs stands for randomized controlled trials. RCTs are a type of research study design that is often…

Q: scuss the impact of the process on society. 6,The end of your paper should include your opinion or…

A: Lactose intolerance is a condition in which the body is unable to fully digest lactose, a sugar…

Q: What does an onion cell look like at interphase at 400x?

A: Cell cycle is a term used to describe a series of intracellular events that take place for the…

Q: Explain the results for each organism. *Nitrate Reduction E. Coli (+) P. aeruginosa (-)

A: Nitrate reduction is a common laboratory test used to determine the ability of microorganisms to…

Q: Using xylitol what happen to in bottle using yeast at 25c and 45c

A: Xylitol is a type of sugar alcohol that is commonly used as a sugar substitute in various foods and…

Q: Describe the role of the cytoskeleton in mitosis specifically regarding the formation of the mitotic…

A: The cytoskeleton is a complex network of protein filaments that provides structure and support to…

Q: Explain why do cells require RNAi

A: The phenomenon of regulating gene expression is known as gene silence. At the level of transcription…

Q: The gap junction channel formed by connexin 43 in cardiac muscle has which of the following…

A: Connexins are a family of transmembrane proteins that form channels called gap junctions. These…

Q: How do the chemical and physical properties of the four main biological molecules affect humans on a…

A: Biological molecules are molecules that are essential to the structure and function of living…

Q: The below graphic depicts what type of kinship chart? ㅇㅅㅇ bilateral unilateral matrilineal…

A: Anthropologists utilize kinship charts to depict a culture's kinship structure without naming…

Q: regarding the USA population are also provided and will be used to determine the "Expected"…

A: According to Hardy Weinberg equilibrium, both allelic and genotypic frequency will remain the same…

Q: The ribosome has 3 tRNA binding binding sites. They are a) EF1, EF2, and EF3 b) 1, 2,…

A: Ribosomes are the molecular machines that synthesize proteins. They are composed of two subunits a…

Q: The arrow in the diagram below indicates the direction of transcription. A BTTT…

A: Q. Ans:- The given image shows the process of transcription. Transcription:- Transcription is a…

Q: 8. How is the Nat channel different from the Na+/K ATPase? Which of the diffusion and which one is…

A: The movement of any substance from its lower concentration towards its higher concentration that is…

Q: Which of the following statements describes a difference between gymnosperms and angiosperms? a.…

A: Plant kingdom is one of 5 kingdom that comprises of green photosynthetic autotrophic plants. It…

Q: Distinguish between gross primary productivity (GPP) and net primary productivity (NPP)

A: Energy flows through natural pathways and biogeochemical cycles. This includes the flow of amounts…

Q: In cells that have a nucleus, the DNA is not able to leave the nucleus. How does the information in…

A: DNA is the genetic material that contains the instructions for all living organisms' development,…

Q: Transduction plates Please find the experimental plates prepared for you from 4 mutati lacZ:: kan…

A: Transduction is when a virus called bacteriophage transfers genetic material from one bacteria to…

Q: Which types of mutations, among those you created in this activity, are more likely to cause the…

A: A mutation is a change that occurs in an organism's DNA sequence. Errors in DNA replication,…

Q: The length of an unknown DNA segment is able to be determined in gel electrophoresis by what method?…

A: The length of an unknown DNA segment can be determined by running it on a gel electrophoresis gel…

Q: The sigma subunit of bacterial RNA polymerase _____. a. binds to a bacterial gene’s promoter b. is…

A: DNA polymerase is an enzyme that catalyzes the formation of new DNA strands from existing DNA…

Q: is a multiple choice question pick the right answer from one of the five choices

A: During the translation process a codon that consists of three nucleotide codes for a single amino…

Q: The doctor prescribes 1000 ml of Dextran 12% within 8 hours for a post-trauma patient. 12gtts/ml is…

A: Dextran is a polysaccharide used in medicine as a blood volume expander. It works by increasing the…

Q: 20 Skipped The Multiple Choice O оо is the brain structure most involved in perceiving interoception…

A: Interoception refers to the perception of internal bodily sensations such as heartbeat, respiration,…

Q: Discuss/Explain in detail, at least 4 different variables that must be considered in any type of…

A: The Neutral Red assay is a laboratory technique used to assess cell viability and cytotoxicity. It…

Q: . search for information and elaborate on rabbits about their Behavior and Habitat 2. please put…

A: Rabbits are interesting mammals that are widely distributed throughout the world. They have unique…

Q: A combinatorial library was screened using microtiter plates for bactericidal antibiotics, compounds…

A: Antibiotics are chemical substances. These are used to retard the growth of microbes. These…

Q: why does fresh milk curdle when vinegar or lemon juice is added ?

A: The building blocks of all living things are proteins. Every cell in the human body contains…

Q: Which process is illustrated in the diagram? NAA 5' Replication Transcription RNA processing…

A: Deoxyribonucleic acid, or DNA, is the genetic material found in humans and the majority of other…

Q: Brown fat, found in small, hibernating animals and infants, has a protein that acts like DNP. We…

A: Brown fat is a special kind of fat in small animals that hibernate and have babies. It helps create…

Q: question--The dairy industry uses enzymes to breakdown lactose in milk, especially for those who…

A: The dairy industry makes milk and things from milk, like cheese and yogurt. It's a big business that…

Question

Analyse the data proved. Which of the E. coli cultures from the regrowth experiment should be used in mutagenesis experiment. Why you selected and why you rejected？

Table 2. The number of cells per milliliter in the overnight cultures prior to dilution in the
regrowth experiment.<
R1 cells/ml R2 cells/ml R3 cells/ml R4 cells/ml R5 cells/ml R6 cells/ml
E. coli
culture
A
B
66664000
56000000 63200000
68800000 69066400
72800000 117866400 63200000
66400000
64800000
78666400 78666400
56266400
76000000
70400000
72800000
71200000
68000000

Growth (OD 600)
2.6
2.4
2.2
Bacterial Growth Curve
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0 1 2 3 4 5 6 7 8 9
T
II I I IIIIIIIII
+
J
I
IT
II
I II
HHA
I
I II
ABC
H
HH
L
RA
H
IT
T T
TT
T
图表区
H-
HH
HOI
HH
HH
HH
HOH
H
R
HH
HH
H
I I
H
HH
HH
HH
HH
10 11 12 13 14 15 16 17 18 19 20
Time (hours)
HI
H
II
21 22
E

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction

VIEW

Step 2: Answer and Explanation

VIEW

Step 3: Answer and Explanation

VIEW

Solution

VIEW

Step by step

Solved in 4 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture would you add to the new media?
procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant
Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features observable under the high-power objective and their movement as seen in the hanging drop preparation
In this step, wash the cells with ice-cold PBS. Drain the PBS, then add ice-cold lysis buffer. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microfuge tube. Maintain constant agitation for 30 minutes at 4°C. Centrifuge in a microcentrifuge at 4°C. You may have to vary the centrifugation force and time depending on the cell type. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet. Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Once you have determined the concentration of each sample, you can freeze them at -20°C or -80°C for later use or prepare for immunoprecipitation or for loading onto a gel. Q4. Why is it necessary to store the prepared lysates in a very low temperature? PLEASE ANSWER Q3 AND Q4
procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.
You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show work
Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
How would you draw out a step in an experiment that included the following? Please help, I am so lost. Metal coupon inoculation (vortex/bead method) and assessment of viable cells by culture to simulate wet fomite or dry touch surface contamination.
If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culture
Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?