Which of the following is true for gene regulation in both bacteria and eukaryotes? Selected answer will be automatically saved. For keyboard navigation, press up/down arrow keys to select an answer. a b Nucleosome structure and histone modifications affect the ability of transcription factors to bind to promoter and enhancer sequences. d Many genes need to be activated only in specific tissues and developmental stages. с Transcription is regulated by trans-acting proteins binding to cis-acting DNA sequences. Genes are often organized into operons, with one mRNA containing coding sequences for several proteins.
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- Which of the following is NOT a reason cells regulate gene expression at a level other than the transcriptional level? Some proteins are only required in part of the cell and transcriptional control will only regulate the mRNA/protein throughout the cell. The core promoter for many genes is the same, so these genes will always be transcribed in the same cells. Differential gene expression in different cell types requires regulation of gene expression at levels other than transcription. Transcription and translation are realtively slow processes, so cells need to regulate gene expression post-transcriptionally if they require a fast change in expression of a gene. Not all cells are transcriptionally active (meaning they do not transcribe any genes), so these cells need to regulate gene expression post-transcriptionally.Consider gene expression in a prokaryotic or bacterial cell. Which of the following is true for transcription in this cell? Select all that apply. During Transcription, the enzyme topoisomerase relaxes negative supercoiling in the DNA strands MRNA can be synthesized by Transcription and at the same time this mRNA can be used for protein synthesis or Translation MRNA has to be processed before leaving the nucleus RNA polymerase requires sigma factor for efficient transcription initiationWhich of the following functions are characteristic of general transcription factors in eukaryotes? Hint: there may be more than one correct response. Be sure to check all that apply. They inhibit RNA polymerase binding to the promoter and begin transcribing. They bind to other proteins or to the TATA box. They are sufficient to allow high levels of transcription. O They initiate and regulate the transcription of genes. O They bind to sequences just after the start site of transcription.
- The diagram below shows the structure of DNA from the level of a gene to a condensed mitotic chromosome. At each of the four locations marked, indicate how a gene may be regulated by selecting from the choices given. Some answer choices may fit into more than one position; however, you should use each answer choice only once. Choices given: Alternative splicing within the gene sequence can lead to different forms of mRNA. A promoter region can be blocked by another protein. Histone deacetylation can prevent DNA from unwinding. A regulatory region can be bound and increase transcription rates.Which of the following is not a general description of the gene expression regulation mechanisms that operate in eukaryotic organisms? transcriptional-level control processing-level control translational level-control replication-level controlsThe most common type of regulation of gene expression occurs at the level of transcription. Name other types of gene regulation for gene expression in eukaryotic cells. Name types of gene regulation unique to eukaryotic cells versus prokaryotic cells.
- e table below with the word that matches the definition in the right column. regulatory proteins (transcription factors) that speed up transcription by helping RNA polymerase bind to a promoter DNA sequences that indicate where transcription of a gene by RNA polymerase begins-typically located upstream from the 5C end Proteins bound to DNA that affect when and how fast a gene is transcribed These factors slow down/shut off transcription either by preventing access to promoters or by keeping RNA polymerase from moving along the DNA strand 3 Regulatory DNA sequénces located upstream or downstream from an associated gene that enhance the transcription rate of that gene when bound to specific proteins 3A sequence of DNA that when bound to a transcription factor blocks the inappropriate effect of an enhancer on a neighboring geneRegulation of transcription is mediated by proteins that first must bind to specific sequences/elements that are present in DNA. Fill in the table below based on your knowledge of the different sequences and proteins involved in the regulation of eukaryotic gene expression. Regulatory sequence Category of Protein that Binds Effect on Transcription of Associated Gene Generic or Gene-Specific Regulation? Core Promoter Element Activator SilencerRegulation of gene expression in eukaryotes can occur: Group of answer choices at the post-transcriptional level, by changes in mRNA stability. all of these at the post-translational level, by modifications to activate proteins. at the transcriptional level, mediated by specific proteins called transcription factors. at the chromatin level, by changes in the state of condensation of chromatin.
- Consider this list (below) of steps involved in transcription. These steps are out of order. TRANSCRIPTION: 1. mRNA travels through a nuclear pore and enters the cytoplasm 2. the mRNA polymerase attaches at the start of a specific gene 3. RNA polymerase reads the gene surface4. a transcription factor bonds to a promoter site5. DNA molecule is unwound 6. a complimentary mRNA is produced What is the correct order of this transcription?Choose all of the statements that describe the benefits of combinatorial control of transcription in a eukaryotic multi-cellular organism. An operon is able to initiate transcription. RNA polymerase has selective access to specific genes for tissue specific effects. RNA reverts to DNA prior to transcription. mRNA synthesis occurs at an accelerated rate.Select four items that are involved in or related to transcriptional control of gene expression (in either pro- or eu-karyotes). -Slide nucleosomes around -control membrane hydrophobicity -methylate DNA -protein denaturation -TFIID and its friends -control mRNA stability -control protein stability -operator/repressor -dynamic instability