Which of the following plates could represent the results of the transformation experiment with PGLO when plated on LB/Amp, if the experiment was successful? Refer to the letters on top of the image. Ignore the letters at the bottom. A в
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- Which of the following plates could represent the results of the transformation experiment with PGLO when plated on LB/Amp, if the experiment was successful? Refer to the letters on top of the image. Ignore the letters at the bottom. A B OB O none of the choices are correctWhat is the purpose of a standard curve for a colorimetric enzyme assay?Why do we need to determine the extinction coefficient in order to calculate the initial velocity in an enzyme kinteic assay experiment?
- Electrophoretic Analysis of DNA via Agarose gel Electrophoresis determine the voltage used (how to determine the voltage? Hint: check on volt/cm).At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.We discussed various assay attributes in class which provide insight into the effectiveness or suitability of a given analytical method. As part of assay development, various attributes were investigated. For each description below, indicate which assay attribute was being evaluated (partial list of attributes include precision, accuracy, specificity, linearity, LOQ/LOD, etc.). The impact of assay temperature and pH on assay results were evaluated to find a suitable range of conditions for the activity assay. Various known concentrations of standard α-AM solutions were evaluated, and the proportionality of the responses were determined. A reference standard of known α-AM (alpha-amylase) concentration was evaluated in triplicate to determine the variability (or scatter) of repeat measurements. Spiked standard of known specific activity and purity was spiked into a sample and the ability for the assay to measure the true biological activity was determined. The impact of various extract…
- During incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…
- Label the NMR and tell what each peak representsFor letter A, pls ILLUSTRATE (create an illustration or drawing) the DILUTION SERIES of the problem just like the sample on the 2nd image. Please read the instructions carefully as I have already posted this twice and the experts just copy-pasted the answers from my first post. I don't want to waste another post question for this one. Again, I NEED AN ILLUSTRATION and not just the computation/explanation through words so I can properly visualize the problem. WILL UPVOTE if I get what I need.In a protocol for DNA sample preparation for agarose gel electrophoresis, what volume of 4X loading buffer must be added to 21 micro L of DNA to obtain a 1X buffer solution?