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- If a 250 ml solution of ethanol in water is prepared with 4 ml of absolute ethanol (100%), what is the dilution factor?Barium sulfate is often used as a medical diagnostic tracer, however, it can only be administered orally, not by IV. Why isn’t it possible to administer this ionic compound intravenously?The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?
- An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.To make 200 ml 0.4XSSC (Saline and Sodium Citrate) solution for FISH post-washing, how many ml 20X SSC solution is needed?
- Beer’s law is strictly obeyed only in dilute solutions. Why?An aqueous solution of 0.2100 g of a mixture containing potassium cyanide and potassium chloride required 14.56 mL of 0.1000 M silver nitrate to produce a faint permanent turbidity; 30.00 mL more of the silver nitrate was added (an excess) and the precipitate of AgCl and AgCN filtered off from the solution. The filtrate and washings were titrated with 13.06 mL 0.1000 M thiocyanate solution. Calculate the percentages of KCl and KCN in the sample. Note: the appearance of faint turbidity indicates the formation of Ag(CN)2-3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL