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Why does penicillin purification use a centrifugal extractor?
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- Briefly discuss the problem of heat management in electrophoretic separations of biomacromolecules.Why is 70% the optimum concentration used in the preparation of ethyl as an antiseptic?A biotechnology company discovers a bacterium isolated from waste industry that produces protease that can operate at high pH. Such protease is useful in making heavy duty detergent that has high pH catalytic condition. Thus, the company decided to produce the protease at large scale using bioreactors. a) Discuss how this is possible when the bacterium is difficult to grow and produce the protease at low level. Suggest any means to overcome these difficulties. b) Suggest what type of bioreactor and mode of culture that is suitable for the production of protease at large scale. Justify your answer.
- why was r. minuta used as the organism of choice for the biotranformation of l-citronellal to l-citronelol instead of p. aeruginosa?With the aid of diagrams, illustrate the biochemical basis of the following: Allopurinol used in the management of gout Hydroxyurea as an antineoplastic agent 6-mercaptopurine, an anticancer agent Trimethoprim, an antibacterial agentSydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.
- What is the difference between Dry and Wet Celloidin Method?Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 IIWhat is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?