You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak in your chromatogram; however, over time you find that a new peak is growing while the original peak is shrinking. What is happening?
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You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak in your chromatogram; however, over time you find that a new peak is growing while the original peak is shrinking. What is happening?
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- You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak in your chromatogra m; however, over time you find that a new peak is growing while the original peak is shrinking. What is happening?Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
- 1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?A GC-MS was used to analyze an unknown sample. Based off the chromatogram, at least how many different compounds were in this sample? Detector Signal u M Retention Time
- Why chromatography is an important tool for analysis of organic compounds?Which one of the following chromatographic techniques is useful in the identification of organic molecules having molecular weights more than 20,000? 1) reverse phase chromatography 2) normal phase chromatography 3) high pressure liquid chromatography 4) gel permeation chromatographyIn molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller molecules
- Look up the boiling point for each of the following compounds then determine the order that they will elute from the column. Use this elution order to identify the compound in each of the 6 standard chromatograms. Once you have identified the compound in each chromatogram, record its retention time. Compound Structure Compound Name Elution Order (1-6) Non polar → Polar Boiling Retention Time (From Chromatograms) Point Propyl acetate H H. H. Butyl acetate H. H. H H. H H. H. H. Methanol H O- Ethanol 2-butanone H H H. H. 4-methyl-2- pentanone H. H. HH H.A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.