You are studying amoebas and paramecia, two different single-celled protozoans. You have at your disposal a standard white light (known as "bright-field") microscope, a fluorescence microscope, a scanning electron microscope, and a transmission electron microscope. For each of the following studies, indicate which type of microscope would be most useful. Items (6 items) (Drag and drop into the appropriate area below) Take movies of unmanipulated cells swimming or crawling in a drop of water. Categories View three- dimensional images of the outer surface of a paramecium, including cilia. bright-field View cytoplasmic streaming (movement of organelles within the cytoplasm) in a paramecium. fluorescence Stain and visualize chromosomes and mitotic spindles in dividing amoebae. Visualize ribosomes in the cytosol and mitochondria of amoebas. scanning electron Use labeled antibodies to locate and make color images of lysosomes in both types of cell. transmission electron

You are studying amoebas and paramecia, two different single-celled protozoans. You have at your disposal a standard white light (known as "bright-field") microscope, a fluorescence microscope, a scanning electron microscope, and a transmission electron microscope. For each of the following studies, indicate which type of microscope would be most useful. Items (6 items) (Drag and drop into the appropriate area below) Take movies of unmanipulated cells swimming or crawling in a drop of water. Categories View three- dimensional images of the outer surface of a paramecium, including cilia. bright-field View cytoplasmic streaming (movement of organelles within the cytoplasm) in a paramecium. fluorescence Stain and visualize chromosomes and mitotic spindles in dividing amoebae. Visualize ribosomes in the cytosol and mitochondria of amoebas. scanning electron Use labeled antibodies to locate and make color images of lysosomes in both types of cell. transmission electron

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Based on what you have learned about the different microscopes, fill in the table below with which one would you use to look at each of the following samples? Explain your answers. Options: Field Scope, dissecting microsope, compound microscope, SEM, or TEM. Subject Microscope Choice Reasoning Live earthworm, 10 cm long Write your answer here Write your answer here Bacterium, 10 µm long Write your answer here Write your answer here Blood from patient suspected of having malaria, a disease caused by a parasite Write your answer here Write your answer here Mouthparts of beetle Write your answer here Write your answer here Mitochondria Write your answer here Write your answer here Blue whale, 30 m long but 500 m away Write your answer here Write your answer here
Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.
Which parts of the modern microscope do you think were absent from Leeuwenhoek’s microscope? Why is it necessary to calibrate the ocular micrometer with each objective? When measuring dimensions of microorganisms, what slide preparation should be used and why? Complete the following for each objective in your microscope: _____ Ocular micrometer divisions = _________ stage micrometer divisions _____ Ocular micrometer divisions = _________1 stage micrometer division = _____mm Microscopic Measurements 1 Arrange the following items by using numbers (smallest) to 8 (largest). ______ AIDS virus ______ rickettsia ______ worm ______ white blood cell (WBC) ______ amoeba ______ protein ______ staphylococci…
Drag the images or descriptions to their corresponding class to test your understanding of various types of microscopes and the images that they produce. Bright-Field Microscope Dark-Field Microscope Scanning Electron Microscope Provides a three- dimensional image when a computer combines multiple optical sections of a specimen stained with fluorescent dye Common light microscope used in the general microbiology lab course Common light microscope used in the general microbiology lab course Fluorescent Microscope Phase Contrast Microscope Confocal Microscope Provides a three- dimensional image when a computer combines multiple optical sections of a specimen stained with fluorescent dye A light microscope that provides brightly colored, highly contrasting, three- dimensional images of live specimens A light microscope that provides brightly colored, highly contrasting, three- dimensional images of live specimens Differential Interference Microscope Transmission Electron Microscope < Prev…
Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would go about solving it. When should the lenses be cleaned? What is the correct way to clean them?
Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles
Read the following scenarios carefully and determine which type of microscope is the most applicable to use. Karen is a scientist at a vast laboratory. She wants to evaluate the structural components of a plant cell, specifically the cell wall. Which is the best for her to use?
WHAT ARE THE USES OF THE FOLLOWING MICROSCOPE AND THEIR RELEVANCE IN THE MEDICAL FIELD? -COMPOUND MICROSCOPE-SIMPLE MICROSCOPE-BRIGHT FIELD MICROSCOPE-LIGHT MICROSCOPE-DARK FIELD MICROSCOPE-PHASE CONTRAST MICROSCOPE -ELECTRON MICROSCOPE-SCANNED PROBE MICROSCOPE-TRANSMISSION ELECTRON MICROSCOPE (TEM)-ATOMIC FORCE MICROSCOPE-DIGITAL MICROSCOPE -DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPE (DIC)-NOMARSKI INTERFERENCE CONTRAST (NIC)-FLUORESCENT MICROSCOPE-CONFOCAL MICROSCOPE-SCANNING ELECTRON MICROSCOPE
What description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.
What are the differences and similarities between Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Please explain in detail.
Using the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?
List two ways that a stereoscopic dissecting microscope differs from a compound microscope