You are studying an unusual enzyme that produces hydronium ions (H3O+) during the course of the reaction. You wish to maintain pH = 8.5 for the enzyme-catalyzed reaction. Which weak acid will serve as the better 0.1 M buffer for the reaction: HEPPS with pKa = 8.0, or, AMPSO with pKa = 9.0?

Chemistry & Chemical Reactivity
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ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
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Chapter17: Principles Of Chemical Reactivity: Other Aspects Of Aqueous Equilibria
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2. You are studying an unusual enzyme that produces hydronium ions (H3O+) during the course
of the reaction.
You wish to maintain pH = 8.5 for the enzyme-catalyzed reaction. Which weak acid will serve
as the better 0.1 M buffer for the reaction: HEPPS with pKa = 8.0, or, AMPSO with pKa = 9.0?
Justify your answer by describing how the buffer “resists” change in pH and the chemical logic
of why you chose the buffer you did.

 

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