Which of the following is/are not essential to carry out the polymerase chain reaction? a. primers b. DNA polymerase c. gel electrophoresis d. high temperature

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Match the terms associated with the polymerase chain reaction with their correct descriptions. Refers to the fact that DNA molecules get longer the more of them there are in the reaction. A. В. Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other. Denaturation C. Incubate the reaction at the optimal temperature for the primers to base-pair with each other. Annealing. D. Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence. Extension. Add a chaotropic agent that destabilizes hydrogen bonding. E. Amplification. F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C). G. Happens after repeated cycles of the temperature change regimen. Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen. Н.

The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction? Group of answer choices a. The number of copies triples (or triplicates). b. The number of copies does not change. c. The number of copies quadruples (or quadruplicates). d. The number of copies doubles (or duplicates). e. The number of copies halves.

Match each enzyme name in the left column with the correct descriptive phrase in the right column. a. Topoisomerase II b. DNA ligase c. DNA polymerase y d. Reverse transcriptase i. Catalyzes most nucleotide incorporations in bacterial DNA replication ii. Cleaves RNA in a DNA-RNA hybrid molecule e. DNA polymerase I f. DNA polymerase II iii. Uses a tRNA primer in synthesis of retroviral DNA iv. Acts through an adenylylated DNA inter- mediate v. Catalyzes formation of a double-strand DNA break vi. Catalyzes mitochondrial DNA replication

Answer 2

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 10, Problem 1MCQ

Which of the following is/are not essential to carry out the polymerase chain reaction?

a. primers
b. DNA polymerase
c. gel electrophoresis
d. high temperature

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 8

Expert Solution & Answer

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

Introduction:

PCR is a technique used to replicate and produce several copies of DNA. PCR has a wide application in research, medicine, agriculture, and many other fields.

Answer 12

Answer to Problem 1MCQ

Correct answer:

Gel electrophoresis is not essential to carry out polymerase chain reaction. Therefore, option (c) is correct.

Option (c) is given as “gel electrophoresis”.

Answer 13

Explanation of Solution

Justify reason for the correct statement:

PCR is a technique used to amplify DNA molecules. This technique involves three steps: denaturation, annealing, and extension. In denaturation, double stranded DNA is heated athigh temperature94 °C and converted into single stranded DNA. After conversion into single stranded, primers get annealed into single stranded DNA. After primer annealing, DNA polymerase enzyme bind to the 3′ end of primer and nucleotides which are complementary to template strand.

After polymerase chain reaction, the resulted fragments are run into gel electrophoresis. Gel electrophoresis is used to separate DNA fragments according to the molecular weight. Thus, gel electrophoresis is not essential to carry out PCR.

Hence, option (c) is correct.

Justify reasons for the incorrect statements:

Option (a) is given as “primers”.

Primers are essential to carry out the polymerase chain reaction. Hence, it is a wrong answer.

Option (b) is given as “DNA polymerase”.

DNA polymerase is important to synthesize DNA fragment. Hence, it is a wrong answer.

Option (d) is given as “high temperature”.

High temperature is required for denaturation of DNA. Hence, it is a wrong answer.

Hence, options (a),(b), and(d) are incorrect.

Answer 14

Conclusion

Gel electrophoresis technique is used after the PCR reaction. Gel electrophoresis technique is used to separate the amplified DNA fragments.

Answer 15

Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYP Ch. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYP Ch. 10.2 - Prob. 5AYP Ch. 10.3 - Prob. 6AYP Ch. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYP Ch. 10.4 - Prob. 9AYP Ch. 10.5 - Outline in general terms the process of DNA...

Which of the following is/are not essential to carry out the polymerase chain reaction? a. primers b. DNA polymerase c. gel electrophoresis d. high temperature

Solution Summary: The author explains that PCR is a technique used to replicate and produce several copies of DNA. Gel electrophoresis is not essential to carry out polymerase chain reaction.

Concept explainers

Videos

Answer to Problem 1MCQ

Explanation of Solution

Want to see more full solutions like this?

Chapter 10 Solutions