Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 10.4, Problem 1MI
Figure 10.6 Electron Movement and Reduction Potentials. Electrons spontaneously move from donors higher on the tower (more negative potentials) to acceptors lower on the tower (more positive potentials). That is, the donor is always higher on the tower than the acceptor. For example, NADH will donate electrons to oxygen and form water in the process. Some typical conjugate redox pairs are shown on the left, and their reduction potentials are given in brackets.
Refer to figure 10.6 and determine the E′0 for NAD+/NADH and coenzyme Q/CoQH2. Suggest a plausible E′0 value for FMN.
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Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase (lecture 22). The enzyme has 3 a-b and 12 ‘c’ subunits. The mitochondrion maintains Df=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well.
How much energy is available (from the proton electrochemical gradient) for ATP synthesis under these conditions (in kJ/mol)?
What [ATP]/[ADP] ratio will be established at steady-state under these conditions?
What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? Remember that full revolution of the crank (gamma subunit) produces 3 ATP.
NH2
'N.
NH
NH
NH2
Benzamidine and Leupeptin are competitive
trypsin inhibitors. They are shown in their
deprotonated forms at high pH.
Modify the above drawings to show the
protonation and charge at pH 7.0
Draw competitive inhibitors for chymotrypsin
based on these structures
IZ
ZI
ZI
.Intramitochondrial ATP concentrations are about 5 mM, and phosphate con-
centration is about 10 mM. If ADP is five times more abundant than AMP,
calculate the molar concentrations of ADP and AMP at an energy charge
of 0.85. Calculate AG for ATP hydrolysis at 37 °C under these conditions.
The energy charge is the concentration of ATP plus half the concentration of
ADP divided by the total adenine nucleotide concentration:
[ATP] + 1/2[ADP]
[ATP] + [ADP] + [AMP]
Chapter 10 Solutions
Prescott's Microbiology
Ch. 10.1 - Figure 10.2 The Relationship of G to the...Ch. 10.1 - Prob. 1CCCh. 10.1 - Prob. 2CCCh. 10.1 - Prob. 3CCCh. 10.1 - Prob. 4CCCh. 10.2 - Why is ATP called a high-energy molecule? How is...Ch. 10.2 - Describe the energy cycle and ATPs role in it....Ch. 10.3 - Prob. 1MICh. 10.3 - Prob. 2MICh. 10.4 - Figure 10.6 Electron Movement and Reduction...
Ch. 10.4 - How is the direction of electron flow between...Ch. 10.4 - When electrons flow from the NAD+/NADH conjugate...Ch. 10.4 - Which among the following would be the best...Ch. 10.4 - In general terms, how is G related to E0? What is...Ch. 10.4 - Name and briefly describe the major electron...Ch. 10.6 - Will an enzyme with a relatively high Km have a...Ch. 10.6 - Prob. 2MICh. 10.6 - Prob. 1CCCh. 10.6 - Prob. 2CCCh. 10.6 - How does enzyme activity change with substrate...Ch. 10.6 - What special properties might an enzyme isolated...Ch. 10.6 - What are competitive and noncompetitive...Ch. 10.6 - How are enzymes and ribozymes similar? How do they...Ch. 10.7 - Figure 10.19 Allosteric Regulation. The structure...Ch. 10.7 - Prob. 2MICh. 10.7 - Define the terms metabolic channeling and...Ch. 10.7 - Define allosteric enzyme and allosteric effector.Ch. 10.7 - Prob. 3CCCh. 10.7 - Prob. 4CCCh. 10.7 - Prob. 5CCCh. 10 - Prob. 1RCCh. 10 - Prob. 2RCCh. 10 - Prob. 3RCCh. 10 - Examine the structures of macromolecules in...Ch. 10 - Examine the branched pathway shown here for the...Ch. 10 - Prob. 3AL
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