Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 18, Problem 18.1CC

Suppose a triplet on the template strand of a piece of DNA has the sequence 5′-ATA-3′. What would be the consequences if the sequence were mutated to GTA? TTA? GCA? Explain your answer in each case.

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Summary Introduction

To determine: The consequences if a sequence 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to GTA.

Introduction: The specific sequences which are present on template strand of DNA (Deoxyribose nucleic acid) in the form of triplet codons will transmit the information for the formation of messenger RNA (Ribose nucleic acid) and proteins.

Explanation of Solution

When a sequence of 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to GTA, it will lead to the formation of messenger RNA having triplet codons UAC in 3' to 5' direction. It occurs because if ATA was present, the resulting messenger RNA would be UAU. Therefore, due to the mutation, UAC will code for amino acid tyrosine similar to UAU because of both UAU and UAC code for amino acid tyrosine.

(b)

Expert Solution
Check Mark
Summary Introduction

To determine: The consequences if a sequence 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to TTA.

Introduction: The specific sequences which are present on template strand of DNA (Deoxyribose nucleic acid) in the form of triplet codons will transmit the information for the formation of messenger RNA (Ribose nucleic acid) and proteins.

Explanation of Solution

When a sequence of 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to TTA, it will lead to the formation of messenger RNA having triplet codons UAA in 3' to 5' direction. It occurs because if ATA was present, the resulting messenger RNA would be UAU. Therefore, due to the mutation, UAA will code for stop codon instead of tyrosine that would be coded by UAU.

(c)

Expert Solution
Check Mark
Summary Introduction

To determine: The consequences if a sequence 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to GCA.

Introduction: The specific sequences which are present on template strand of DNA (Deoxyribose nucleic acid) in the form of triplet codons will transmit the information for the formation of messenger RNA (Ribose nucleic acid) and proteins.

Explanation of Solution

When a sequence of 5ʹ-ATA-3ʹ on template strand of DNA has been mutated to GCA, it will lead to the formation of messenger RNA having triplet codons UGC in 3' to 5' direction. It occurs because if ATA was present, the resulting messenger RNA would be UAU. Therefore, due to the mutation, UGC will code for amino acid cysteine instead of tyrosine that would be coded by UAU.

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Suppose a single-nucleotide polymorphism occurred in the original strand to make the change shown below. Would this affect the resulting protein? Explain. This is the original strand TGG CGA GAG GGG AGC AGC TGG TAT CGC TGG GCC CAA AGG TCT TCA GGG AAT GCC This is the strand with the SNP. (The change is shown in red.) TGG CGA GAG GGG AGC AGC TAG TAT CGC TGG GCC САА AGG TCT TCA GGG AAT GCC Suppose a different single-nucleotide polymorphism occurred in the original strand to make the change shown below. Would this affect the resulting protein? Explain. This is the original strand AGG TCT TCA GGG AAT GCC TGG CGA GAG GGG AGC AGC TGG TAT CGC TGG GCC САА This is the mutated strand. (The change is shown in red). AGG TCT TCA GGG AAT GCC TGG CGA GAG GGA AGC AGC TGG TAT CGC TGG GCC CAA
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Which of the following set(s) of primers a-d could you use to amplify the following target DNA sequence, which is part of the last protein-coding exon of the CFTR gene? Explain briefly. (Note: The three dots represent the body of the region to be amplified, whose beginning and end are only being shown.) 5' GGCTAAGATCTGAATTTTCCGAG . TTGGGCAATAATGTAGCGCCTT 3' 3' CCGATTCTAGACTTAAAAGGCTC . AACCCGTTATTACATCGCGGAA 5' a. 5' GGAAAATTCAGATCTTAG 3'; 5' TGGGCAATAATGTAGCGC 3' b. 5' GCTAAGATCTGAATTTTC 3'; 3' ACCCGTTATTACATCGCG 5' c. 3' GATTCTAGACTTAAAGGC 5'; 3' АССCGTTATTАСАТСGCG 5 d. 5' GCTAAGATCTGAATTTTC 3'; 5' TGGGCAATAATGTAGCGC 3'
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