Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 7.2, Problem 1MI
MICRO INQUIRY
Why is it important that the origin of replication migrate to the center of the cell prior to replication?
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I. Compare how Bacteria, Archaea, and Eukaryotes differ on each of the following aspects of
DNA replication:
1. How do the number and types of DNA polymerase differ between these three groups?
2. How does the physical location in the cell of DNA replication differ between these three
groups?
3. Are there differences between these three groups in the timing of when DNA replication
occurs in cells?
4. How does origin of replication differ in terms of number and location between these three
groups?
Application/ Analysis
Explain how the anti-parallel structure of DNA predicts its replication mechanism.
Identify the major and minor groove of DNA and explain why they are there.
Differentiate between semiconservative, conservative, and dispersive replication.
Interpret a diagram of a bi-directional replication fork and correctly determine strand polarity and fork direction.
Preforming a "blue-white screen"
3) Would bacteria that have taken up a plasmid into which a DNA fragment has been inserted, form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.
Chapter 7 Solutions
Prescott's Microbiology
Ch. 7.1 - MICRO INQUIRY In addition to chromosomes, what...Ch. 7.2 - MICRO INQUIRY Why is it important that the origin...Ch. 7.2 - MICRO INQUIRY What would be the outcome if FtsZ...Ch. 7.2 - MICRO INQUIRY Which step in the development of...Ch. 7.2 - Retrieve, Infer, Apply 1. Describe the three...Ch. 7.2 - Retrieve, Infer, Apply 2. How does the bacterial...Ch. 7.2 - Retrieve, Infer, Apply 3. Do you think MinCDE...Ch. 7.2 - Retrieve, Infer, Apply 4. Do you think Spiroplasma...Ch. 7.3 - What elements of the Sulfolobus spp. cell cycle...Ch. 7.3 - Many archaea have genes encoding an FtsZ...
Ch. 7.4 - MICRO INQUIRY Identify the regions of the growth...Ch. 7.4 - Retrieve, Infer, Apply Define microbial growth.Ch. 7.4 - Retrieve, Infer, Apply Describe the phases of the...Ch. 7.4 - Why would cells that are vigorously growing when...Ch. 7.4 - Calculate the growth rate constant and generation...Ch. 7.4 - Suppose the generation time of a bacterium is 90...Ch. 7.5 - What is the difference between halophilic and...Ch. 7.5 - Why do facultative anaerobes grow best at the...Ch. 7.5 - Retrieve, Infer, Apply 1. How do microorganisms...Ch. 7.5 - Retrieve, Infer, Apply 2. Define water activity...Ch. 7.5 - Prob. 1.3CCCh. 7.5 - Retrieve, Infer, Apply 1. Define pH, acidophile,...Ch. 7.5 - Retrieve, Infer, Apply Classify each of the...Ch. 7.5 - Retrieve, Infer, Apply 3. Describe the mechanisms...Ch. 7.5 - Retrieve, Infer, Apply 1. What are cardinal...Ch. 7.5 - Prob. 3.2CCCh. 7.5 - Prob. 3.3CCCh. 7.5 - Prob. 3.4CCCh. 7.5 - Retrieve, Infer, Apply Describe the five types of...Ch. 7.5 - Retrieve, Infer, Apply What are the toxic effects...Ch. 7.5 - Retrieve, Infer, Apply Where would you expect to...Ch. 7.5 - Retrieve, Infer, Apply List the types of...Ch. 7.5 - Prob. 5.3CCCh. 7.5 - Prob. 5.4CCCh. 7.6 - MICRO INQUIRY What biomolecules make up the...Ch. 7.6 - Prob. 1CCCh. 7.6 - Prob. 2CCCh. 7.6 - Prob. 3CCCh. 7.6 - Retrieve, Infer, Apply What is quorum sensing?...Ch. 7.6 - Retrieve, Infer, Apply How is the communication...Ch. 7.7 - Prob. 1CCCh. 7.7 - What are peptones, yeast extract, beef extract,...Ch. 7.7 - Retrieve, Infer, Apply Describe four ways in which...Ch. 7.7 - What are pure cultures and why are they important?...Ch. 7.7 - Retrieve, Infer, Apply It is known that microbial...Ch. 7.7 - Retrieve, Infer, Apply How might an enrichment...Ch. 7.8 - Why is it important to have no more than about 250...Ch. 7.8 - Briefly describe each technique by which microbial...Ch. 7.8 - Prob. 2CCCh. 7.8 - Prob. 3CCCh. 7.8 - For each of the following, which enumeration...Ch. 7.9 - Prob. 1MICh. 7.9 - Prob. 1CCCh. 7.9 - Prob. 2CCCh. 7.9 - Prob. 3CCCh. 7 - Prob. 1RCCh. 7 - Prob. 2RCCh. 7 - Prob. 3RCCh. 7 - Prob. 4RCCh. 7 - Prob. 5RCCh. 7 - Prob. 6RCCh. 7 - As an alternative to diffusible signals, suggest...Ch. 7 - If you wished to obtain a pure culture of bacteria...Ch. 7 - Prob. 3ALCh. 7 - Suggest one specific mechanism underlying the...Ch. 7 - Consider cell-cell communication: bacteria that...Ch. 7 - Suppose you discovered a new bacterial strain from...Ch. 7 - Prob. 7ALCh. 7 - Prob. 8ALCh. 7 - Prob. 9AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) Explain semi-conservative modelmof DNA replication b) discuss initiation in DNA replication and explain why it is a tightly controlled process.arrow_forwardDiscuss about conservative replication ?arrow_forwardClearly diagram rolling circle replication. Add 5’ and 3’ and show the direction of replication with arrows. Include the part that looks like “Okazaki fragments.”arrow_forward
- Correct order ib which the following enzynes would operate to fix a damaged nucleotide in a human gene. a) nuclease, DNA polymerase, RNA primase b) helicase, DNA polymerase, DNA ligase c) DNA ligase, nuclease, helicase d) nuclease, DNA polymerase, DNA ligasearrow_forwardWhat are nanopores used for and tell How does DNA bind to the nanopore?arrow_forwardExplain the semiconservative mode of replication ?arrow_forward
- BIOCHEMISTRY Will material of extraction membrane disturb enzyme in reverse transcription, exonuclease and PCR?arrow_forward2) Replicating structures in DNA can be observed in the electron microscope. Regions being replicated appear as bubbles. a) How many replication forks are present? b) Assuming bidirectional replication, how many origins of replication are active in this DNA molecule? c) Assuming that all replication forks move at the same speed, which origin of replication was activated first (left, middle or right)? Why?arrow_forwardFigure 6-7 CONDITION (A) bacteria grown in light medium (B) bacteria grown in heavy medium TRANSFER TO LIGHT MEDIUM (C) bacteria grown an additional 1 hour in light medium RESULT centrifugal force centrifugal force centrifugal force INTERPRETATION AVIV AVVY AVVI light DNA molecules AMM heavy DNA molecules OR WW DNA molecules of intermediate weight In the original Meselson-Stahl experiment shown above bacteria were cultured in 15N-containing "heavy" medium and thenarrow_forward
- SCi 7. Superhelical Density Bacteriophage A infects E. coli by wi integrating its DNA into the bacterial chromosome. The suc- cess of this recombination depends on the topology of the E. coli DNA. When the superhelical density (ơ) of the E. coli DNA is greater than -0.045, the probability of integration is <20%; when o is less than -0.06, the probability is –70%. the at lor 10 sic Plasmid DNA isolated from an E. coli culture is found to have is a length of 13,800 bp and an Lk of 1,222. Calculate o for this DNA and predict the likelihood that bacteriophage A will be 14 able to infect this culture. sisarrow_forwardQ. What might happen to single ssDNA if this protein was not present during replication?arrow_forward"Hybridization of a single-stranded DNA molecule attached to a fluorophore with a preparation of metaphase chromosomes that have been partially denatured" is a description of which laboratory method?arrow_forward
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