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Practice calculating dilutions using the following problems.
- Dilution:
To determine:
The serial dilution in the given samples.
Introduction:
The stepwise dilution of a solution is termed as a serial dilution. Generally, the dilution factor at each stage of serial dilution is constant and leads to geometric progression in a logarithmic manner of the concentration of the sample. Serial dilutions are used to dilute the given substance at a very high level and serial dilutions are also used in the formation of concentration curves.
Explanation of Solution
The samples where serial dilution is occurring is shown below:
1 ml of the sample is mixed in 99ml solvent. So, the total solution is of 100 ml.
Hence, in tube 1 the serial dilution is of 1:100.
Now, 1 ml of the sample from tube 1 is poured to 99 ml solvent in tube 2. So, the total volume of the solution in tube 2 becomes 100 ml.
Hence, in tube 2 the serial dilution is now =
Now, 1 ml of the sample from tube 2 is poured to 9 ml solvent in tube 3. So, the total volume of the solution in tube 3 becomes 10 ml.
Hence, in tube 3 the serial dilution is now =
Now, 1 ml of the sample from tube 3 is poured to 9 ml solvent in tube 4. So, the total volume of the solution in tube 4 becomes 10 ml.
Hence, in tube 4 the serial dilution is now =
The samples where serial dilution is occurring is shown below:
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