Enzyme

substrate concentration, the enzyme is working at “maximum efficiency.” With a concentration at 40, it produced 2,339 products. 2. The maximum velocity of a reaction is reached when the active sites are almost continuously filled. Increased substrate concentration after this point will not increase the rate. The reaction rate increases as substrate concentration is increased. It will soon level off though. 3. When the concentration is at low substrate, most of the enzyme molecules are not filled

Galactocerebrosidase enzyme (GALC) is an enzyme with very low activity, it is a lysosomal acid hydrolase or a lysosomal catabolism that stimulate the removal of a monosaccharide sugar which is galactose that is found in galactosylceramide or galactocerebroside (GalCer) or anything descendant from ceramide. Galactocerebroside is a marker for oligodendrocytes in the brain, regardless of whether they shape myelin. It is encoded by a specific gene which is (GALC) which is is formed in the endoplasmic

toothpicks/pop beads over time relates to enzymes building up or breaking down substrates. It was then hypothesized that if the method of breaking toothpicks changes, then the amount of toothpicks broken will decrease as it gets harder. This hypothesis was supported by the data because it proves that as it grew harder to break the toothpick, less would be broken over 90 seconds. These experiments demonstrate that as it becomes more difficult for an enzyme to break down a substrate, it will take a

by something called Enzymes. Enzymes are basically catalyst that are organic which are made up of hundreds of amino acids and are pertained as fairly large (Biology Department, n.d, p.34). Similar to other catalyst, enzymes have the capability of fulfilling their function without allowing themselves to be consumed as well as disfigured. Enzymes can also carry on their function without changing the chemical equilibrium between reactants and products (Cooper GM, 2000). Enzymes main function is to help

temperature and pH were measured on the catalytic ability of the enzyme catechol oxidase (also known as tyrosinase, diphenol oxidase, or polyphenal oxidase). Enzymes are defined as catalysts in biological systems that lower that energy of activation or Ea of a reaction. When a substrate bonds to a the active site of an enzyme, this forms an enzyme-substrate complex. An enzyme substrate complex consists of one or more substrates bonded to the enzymes active site, which changes shape slightly when bonded to

World Connections/Discussion In both experiments, there was one environment that the enzyme worked best it. The graphs show that in the salt experiment 4% salt concentration was the optimum environment for the enzyme, and pH 6 buffer solution was the optimum pH environment for the enzyme. In the other environments the enzyme did not work as fast, therefore not creating as much product. The hypothesis was that the enzyme would work the most efficiently in the most neutral environment. The data does support

approach described above is novel and original because we are integrating the high level of programmability of DNA with natural catalysts, which are very significant to progress in many different scientific fields. Such integration enables coupling of enzymes to circuits that are capable of decision making and self-regulation, leading to an unprecedented level of control of catalytic reactions. To achieve our objectives, we will develop methods to systematically design, model and test both dynamic and

pH levels effect on Enzyme Activity and Concentration Abstract: pH level may affect enzyme activity and the performance of the enzyme which is crucial in order for the enzyme to properly function in our biological systems. We investigated the relationship between pH level and rate of enzyme function at that certain pH level. The enzyme concentration was recorded at varying levels of pH using the enzyme Lactase, substrate ONPG, and phosphate buffer in a spectrophotometer set at wavelength 405nm. As

Enzymes are protein molecules composed of amino acids and are made by the living cell. These molecules provide energy for the organism by catalyzing various biochemical reactions. If enzymes were not present in cells, most of the chemical reactions would not proceed at measurable rates at the temperatures of living systems. Therefore, we can say that being involved as catalysts is the main and most important role of enzyme in any organism. Many reactions that are thermodynamically favored do not

concentration effect enzyme activity I. Introduction Enzymes act as a catalyst in nature. They lower the activation energy of the chemical reaction. They can speed up or slow down depending on the surrounding conditions. In this lab, the depending variable is the color change due to the reaction between oxygen and guaiacol, and the independent variable is the time in minutes that take the reaction to occur. The enzymes being used in this experiment is peroxidase. This enzyme is used in a biological