Molecular biology

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    Biology is the science that studies the nature of life. Unlike others, biology is the closest branch of science to the human way of life. Biology has many specific areas of focus, for example, ecology, entomology, physiology, and molecular biology. In the past, molecular biology was ignored in many laboratories because it requires expensive tools. Until the instruments become cheaper and the chemicals become more affordable, the importance of molecular biology will not rise. In studying entomology

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    patients and my fear of it, I was thinking about a new way to find a drug that can treat it without major side effects. That's why I planned to study pharmacy to be aware of cancer biology and latest approaches for it's treatment. Then I decided to continue my studies and to get my masters in the field of Molecular Biology so I can perform researches and try to find a new treatment for this disease. since my financial abilities is not well established, I was searching how to win a scholarship and what

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    I developed a strong interest in basic sciences, especially biochemistry and molecular biology. After my medical training, I joined the Ph.D. program at the University of Houston to pursue my interest in molecular biology. For my doctoral research, I investigated the role of steroid hormones in prostate cancer and central nervous system development. In addition to learning the fundamentals of molecular biology, I gained experience in cloning, polymerase chain reaction, liquid chromatography

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    I looked up each molecular biologist and used Wikipedia to get a brief overview of each individual. Dr. Lydia Villa-Komaroff came my eye because she was part of a team that discovered how bacterial cells can be used to make insulin and I wanted to know more on how the bacteria was able to do this. Villa-Komaroff attended University of Washington in Seattle, majoring in chemistry (United Neighborhood Houses). She was struggling in chemistry and went to her adviser for assistance. Her adviser told

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    *Running title: Isolation and Characterization of Avian Lactate Dehydrogenase To whom correspondence should be addressed Sylvia DaoudKinze and James Proestes, Department of Biochemistry, Portland State University Professor, Portland Oregon, 97207-0751; E-mail: sylviakinzie@gmail.com and proestoj@gmail.com ------------------------------------------------- Keywords: Lactate, Dehydrogenase, Avian, Bradford Assay, Affinity Column Background: Lactate Dehydrogenase also known as LDH is an important

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    Molecular Genetic Experiment: Exploring the Expression of an Auxin Induced Gene pCNT103, a Constitutive Gene GapC and a Cytokinin Induced Cig1 Gene in the DNA and RNA Extracts of Differentiated Shoot, Root, and Callus Tissue of Nicotiana tabacum Demaris Gonzalez Genetics Lab Professor Kamil Starczak March 23rd , 2017 Abstract: The experiments purpose was to understand and observe the gene expressions in the genes pCNT103, cig1 and GapC in the shoot, root and callus tissues of the tobacco

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    Introduction The central dogma of molecular biology is widely known as the transcription of DNA to RNA, and then the translation of RNA to protein. In these past few experiments, the students looked at mRNA, treated them with PMA and DMSO. The students then performed reverse transcriptase on their mRNAs in order to convert them into cDNA, which is more stable. After that, the students then performed PCR on their cDNA to zero in on the gene of choice. Those genes were then run through gel electrophoresis

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    Two major breakthroughs in molecular biology, DNA fingerprinting discovered by Professor Sir Alec Jeffreys and Polymerase Chain Reaction (PCR) invented by Kary Mullis, were exceptionally useful in criminal investigation. These DNA-based technologies can aid in the fight against crime because they can be used to distinguish a small set of DNA variations that is extremely probable to be different and unique in all unrelated individuals, which can be applied directly to problems of human identification

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    SIB2003 Molecular Biology Practicals Manipulation and analysis of DNA using standard Molecular Biology Techniques. During the course of the next three practical classes you shall be performing a number of techniques in order to isolate and manipulate DNA from bacteria. The practicals are spread over three sessions, the techniques that you will perform are indicated below: Practical 1 Isolation of plasmid DNA from three cultures of E.coli using a method known as the alkaline lysis method. Practical

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    transcriptase polymerase chain reaction, also known as RT-PCR, has been recognized as a reliable, accurate, and sensitive method for quantifying gene transcription. Polymerase chain reaction, also known as PCR, is considered an essential tool in molecular biology that allows for the amplification of nucleic acid sequences. Specifically, the three main consecutively repeating steps in PCR are denaturation, annealing, and elongation. If the reaction runs with 100% efficiency, there will be a two-fold increase

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